FLUORESCENCE MICROSCOPY OF URINARY BLADDER WALL

目的是鉴定各种荧光生化和 使用荧光的膀胱组织中的形态学成分 显微镜 膀胱组织的冷冻切片用 荧光显微镜 将连续切片染色并由 病理学家 在370 nm和400 nm的激发下，浸润性癌症具有 除了看起来分散的微小荧光外， 胶原碎片和偶尔的炎性细胞。 对于其他 组织诊断，上皮细胞没有或检测不到 荧光。 粘膜下层中的胶原纤维呈蓝色 字符串，是主要的荧光团，虽然偶尔 黄绿色的斑点似乎是炎症细胞， 出现 浸润性癌症缺乏荧光 观察到非常弱的荧光光谱。 没有 根据观察结果，预期不会出现上皮荧光 早期的结肠异型增生的隐窝细胞荧光。 图像中 CIS组粘膜下层荧光胶原表达减少， 股，但鉴于最近的cyanide结果，相应的 染色的载玻片需要仔细检查水肿或出血 这可能在粘膜下层的变化中起作用。 分析和 对这些显微镜图像的解读仍在进行中。

The objective is identify various fluorescent biochemical and morphological components in bladder tissues using fluorescence microscopy. Frozen sections of bladder tissue were imaged with a fluorescent microscope. Serial sections were stained and reviewed by a pathologist. At 370 nm and 400 nm excitation, invasive cancer had little fluorescence other than what appeared to be scattered tiny collagen fragments and occasional inflammatory cells. For the other tissue diagnoses, epithelium had either no or undetectable fluorescence. Collagen strands in the submucosa, appearing as blue strings, were the primary fluorophore, although occasional yellowish-green spots that seem to be inflammatory cells also appeared. The lack of fluorescence of the invasive cancer explains the much weakened fluorescence spectra observed. The absence of epithelial fluorescence was not expected based on the observation earlier of dysplastic crypt cell fluorescence in the colon. In images ta ken of CIS, the submucosa exhibited fewer fluorescencing collagen strands, but in view of recent cystectomy results, the corresponding stained slides need to be closely checked for edema or hemorrhage which might play a role in this change in the submucosa. Analysis and interpretation of these microscope images is still ongoing.