REFLECTANCE SPECTROSCOPY FOR DIAGNOSIS OF PRE CANCEROUS CHANGES IN COLON

我们开发了一种新的基于光谱反射的方法 检测生物组织中与 癌症形成的早期阶段。我们使用了基于漫反射的设置 反射光谱仪在临床环境中收集数据 用于测量反射率数据的白光激励由 紧凑型4J/脉冲氙弧灯(EG&G，FX-249型)。那盏灯 提供10毫秒的脉冲宽度。激励光束被耦合到一个 200 fm芯径光纤(0.22 nA)。通过使用PULSE 白光光源的激发和反射率的门控检测 信号时，照明源的背景信号被最小化。 数据采集由486笔记本微型计算机控制。这个 研究采用收集住院期间结肠反射光谱的方法 内窥镜检查程序并使用物理模型进行处理。这个 模型考虑了光在氧-和-存在时的传播 脱氧血红蛋白和分散在胶原纤维上。这项研究有 结果表明：(A)有可能测量 利用物理模型描述活体中的人结肠组织 组织漫反射，以及(B)测量的光学特性 可以与生化和形态组成相关联 这对疾病的检测很有用。

We developed a new spectroscopic reflectance-based method of detecting alterations in biological tissues which are related to the early stages of cancer formation. We used setup based on diffuse reflectance spectrometer to collect data in the clinical settings The white light excitation for measured reflectance data was provided by a compact 4J/pulse xenon arc lamp (EG&G, model FX-249). The lamp provides a pulse width of 10 ms. Excitation beam was coupled into a 200 fm core diameter optical fiber (0.22 NA). By using pulsed excitation of white light source and gated detection of reflectance signal, background signal from illumination source was minimized. Data acquisition is controlled by a 486-notebook microcomputer. The study employs collection of reflected spectra in colon during hospital endoscopy procedures and processing them using physical model. The model takes into account propagation of light in presence of oxy- and de-oxy- hemoglobin and scattering on coll agen fibers. This study has shown that: (a) it is possible to measure the optical properties of human colon tissue in vivo by employing a physical model describing tissue diffuse reflectance, and (b) the optical properties measured can be correlated with the biochemical and morphological composition of tissue, which can be useful for the detection of disease.