REFLECTANCE SPECTROSCOPY FOR DIAGNOSIS OF PRE CANCEROUS CHANGES IN COLON

反射光谱诊断结肠癌前病变

• 批准号: 6314208
• 负责人: GEORGE ZONIOS
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314208
• 关键词: biomedical resource; human tissue; model design /development; technology /technique

We developed a new spectroscopic reflectance-based method of detecting alterations in biological tissues which are related to the early stages of cancer formation. We used setup based on diffuse reflectance spectrometer to collect data in the clinical settings The white light excitation for measured reflectance data was provided by a compact 4J/pulse xenon arc lamp (EG&G, model FX-249). The lamp provides a pulse width of 10 ms. Excitation beam was coupled into a 200 fm core diameter optical fiber (0.22 NA). By using pulsed excitation of white light source and gated detection of reflectance signal, background signal from illumination source was minimized. Data acquisition is controlled by a 486-notebook microcomputer. The study employs collection of reflected spectra in colon during hospital endoscopy procedures and processing them using physical model. The model takes into account propagation of light in presence of oxy- and de-oxy- hemoglobin and scattering on coll agen fibers. This study has shown that: (a) it is possible to measure the optical properties of human colon tissue in vivo by employing a physical model describing tissue diffuse reflectance, and (b) the optical properties measured can be correlated with the biochemical and morphological composition of tissue, which can be useful for the detection of disease.

我们开发了一种新的基于光谱反射率的方法， 检测生物组织中的改变，所述改变与 癌症形成的早期阶段。 我们使用基于漫反射的设置 在临床环境中收集数据的反射光谱仪 用于测量反射率数据的白色光激发由 紧凑型4J/脉冲氙弧灯（EG&G，FX-249型）。 灯 提供10 ms的脉冲宽度。 200 μ m芯径光纤（0.22 NA）。 利用脉冲 白色光源的激发和反射率的选通检测 信号，来自照明源的背景信号被最小化。 数据采集由486笔记本微机控制。 的 一项研究采用了在住院期间收集结肠中的反射光谱 内窥镜检查程序并使用物理模型处理它们。 的 模型考虑了光在氧存在下的传播 脱氧血红蛋白和分散在科尔阿根纤维上。 本研究 表明：（a）可以测量的光学性能 人体结肠组织在体内通过采用物理模型， 组织漫反射率，和（B）测量的光学性质 可以与生化和形态组成相关 这对疾病的检测很有用。