MOTIONAL PROPERTIES OF HYDROCARBON NITROXIDES IN PHOSPHOLIPID BILAYERS

磷脂双层中烃类氮氧化物的运动性质

• 批准号: 6120679
• 负责人: PHILIP D MORSE
• 金额: $ 0.27万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120679
• 关键词: biological products; biomedical resource; drug /agent; human tissue; lipids

Partitioning of small piperidine-based nitroxides (2,2,6,6-tetramethyl-piperidine-N-oxyl-4-R) into lipid bilayers followed by examination of these systems through electron paramagnetic resonance (EPR) spectroscopy has provided useful information concerning the structure and function of the cell membrane. However, there are classes of membranes which do not readily lend themselves to partitioning studies by these nitroxides (for example, the red blood cell membrane). Additionally, piperidine-based nitroxides are typically spherically symmetric and do not provide much information about directional anisotropy in the lipid regions of membranes. Therefore we have developed a panel of oxazolidine-derived nitroxides of increasing chain length to both permit partitioning studies in formerly inaccessable membrane systems and to provide structural and hence motional anisotropy. We describe the synthesis, derived from the method of Keana et al, of this series of nitroxide spin label probes and t heir characterization by IR, NMR, and mass spectrometry. Experimental results on partitioning and motion by X- and W-band EPR of these nitroxides in lipid bilayers (liposomes) of dipalmitoylphosphatidylcholine and phospholipid mixtures have also been obtained and analyzed. This is a continuing project.

小分子哌啶基氮氧自由基的分配 （2，2，6，6-四甲基-哌啶-N-氧基-4-R）成脂双层 然后通过电子顺磁共振技术检查这些系统， 电子顺磁共振（EPR）光谱提供了有用的信息 关于细胞膜的结构和功能。 然而，在这方面， 有几类膜不容易使它们自己 通过这些氮氧化合物的分配研究（例如，红血 细胞膜）。 此外，基于哌啶的氮氧化物是 通常是球对称的，并不提供太多的信息 关于细胞膜脂质区域的方向各向异性。 因此，我们开发了一组恶唑烷衍生的氮氧化物 增加链长，以允许在 以前无法进入的膜系统，并提供结构和 因此是运动各向异性。 我们描述的合成，来自 Keana等人的方法，这一系列的氮氧自旋标记 探针，并通过IR、NMR和质谱对其进行表征。 X波段和W波段EPR的分割和运动实验结果 这些氮氧化物的脂质双层（脂质体）， 二棕榈酰磷脂酰胆碱和磷脂混合物也 得到并分析。 这是一个持续的项目。