EFFECT OF NITROXIDES ON CELL GROWTH & DEVELOPMENT

细胞生长需要对许多细胞事件进行精确的计时 如DNA复制、染色质形成和染色体 对齐。此过程中的多个检查点可确保 导致细胞分裂的事件以正确的顺序发生 (即，细胞分裂不会在染色体分离之前发生)。 氧自由基可能在这种同步性中发挥作用，可能是因为 信号分子。氮氧化物是众所周知的超氧化物歧化酶 模拟过氧化氢酶，在某些过渡金属存在的情况下 模仿学。因此，它们可能会干扰细胞的同步性 组织。我们发现BHK(小仓鼠肾脏)细胞的生长是 加速0.1至0.2 mm，抑制0.4至1.0 mm Tempoll (2，2，6，6-四甲基哌啶-N-氧基-4-醇)。 在0.4至1.0 mM的范围内，坦波尔不会杀死细胞。这 提示BHK细胞的生长可能受氧自由基的调节。 这项工作依赖于近距离开发的一个软件包 与IERC协作；该软件不断更新以 适应这个项目的需要。

Cell growth necessitates precise timing of a number of cell events such as DNA replication, chromatin formation, and chromosome alignment. A number of checkpoints in this process acts to insure that events leading to cell division occur in the proper sequence (i.e., cell division doesn't occur before chromosome segregation). Oxygen radicals may play a role in this synchrony, perhaps as signaling molecules. Nitroxides are well-known superoxide dismutase mimetics and, in presence of some transition metals, catalase mimetics. Therefore, they may interfere with the synchrony of cell division. We show that growth of BHK (baby hamster kidney) cells is accelerated by 0.1 to 0.2 mM and inhibited by 0.4 to 1.0 mM Tempol (2,2,6,6-tetramethyl piperidine-N-oxyl-4-ol) in the growth media. Tempol in the range of 0.4 to 1.0 mM does not kill the cells. This suggests that growth of BHK cells may be mediated by oxygen radicals. This work relies on a software package developed in a close collaboration with the IERC; the software is constantly updated to accommodate the needs of this project.