EFFECT OF NITROXIDES ON CELL GROWTH & DEVELOPMENT

氮氧化物对细胞生长的影响

• 批准号: 6120710
• 负责人: PHILIP D MORSE
• 金额: $ 0.05万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120710
• 关键词: animal tissue; biomedical resource; environmental health; technology /technique

Cell growth necessitates precise timing of a number of cell events such as DNA replication, chromatin formation, and chromosome alignment. A number of checkpoints in this process acts to insure that events leading to cell division occur in the proper sequence (i.e., cell division doesn't occur before chromosome segregation). Oxygen radicals may play a role in this synchrony, perhaps as signaling molecules. Nitroxides are well-known superoxide dismutase mimetics and, in presence of some transition metals, catalase mimetics. Therefore, they may interfere with the synchrony of cell division. We show that growth of BHK (baby hamster kidney) cells is accelerated by 0.1 to 0.2 mM and inhibited by 0.4 to 1.0 mM Tempol (2,2,6,6-tetramethyl piperidine-N-oxyl-4-ol) in the growth media. Tempol in the range of 0.4 to 1.0 mM does not kill the cells. This suggests that growth of BHK cells may be mediated by oxygen radicals. This work relies on a software package developed in a close collaboration with the IERC; the software is constantly updated to accommodate the needs of this project.

细胞生长需要精确的细胞事件的时间 如DNA复制、染色质形成和染色体 对齐。 此过程中的许多检查点可确保 导致细胞分裂的事件是按照正确的顺序发生的 (i.e.,细胞分裂在染色体分离之前不会发生）。 氧自由基可能在这种同步中发挥作用， 信号分子 氮氧自由基是众所周知的超氧化物歧化酶 模拟物和在某些过渡金属存在下的过氧化氢酶 拟态 因此，它们可能会干扰细胞的同步 师. 我们发现BHK（婴儿仓鼠肾）细胞的生长是 0.1 - 0.2 mM加速，0.4 - 1.0 mM Tempol抑制 （2，2，6，6-四甲基哌啶-N-氧基-4-醇）。 0.4至1.0 mM范围内的Tempol不会杀死细胞。 这 表明BHK细胞的生长可能是由氧自由基介导的。 这项工作依赖于一个软件包， 与IERC合作;软件不断更新， 满足这个项目的需要。