NMR OF TRANSITION BETWEEN DIMER & TETRAMER OF EK18, MUTANT OF TRP REPRESSOR

二聚体之间转变的 NMR

• 批准号: 6281618
• 负责人: CATHERINE A ROYER
• 金额: $ 1.36万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281618
• 关键词: biological products; biomaterials; biomedical resource; nuclear magnetic resonance spectroscopy; proteins

EK18 is a mutant of trp repressor and is cal a "super-repressor" since it represses the transcription of genes responsible for typtophan synthesis at a lower level of tryptophan molecules in the cell. Its molecular weight is about 12.5 kDaltons per monomer and it exists primarily as a dimer. However, when the salt concentration is lowered, or the protein concentration is increased, oligomerization occurs between dimers. The protein-protein interaction seems to play a role in the mechanism of repression. In addition to the corepressor concentration, this oligomerization may act to fine tune the gene regulation. Several studies have been done using fluorescence spectroscopy to investigate the oligomerization, but no structural studies have been done to date. NMR studies of this oligomerization will tell how the two dimers interact (i.e., whether the tetramerization is specific or nonspecific, or whether is it symmetric or unsymmetric).

EK18是TRP阻遏物的突变体，是Cal的“超级防压器” 由于它压抑了负责的基因的转录 在较低水平的色氨酸分子中的Typtan合成 细胞。 它的分子量约为每个单体的12.5 kdaltons，它 主要作为二聚体存在。 但是，当盐浓度为 降低或蛋白质浓度升高，寡聚化 发生在二聚体之间。 蛋白质 - 蛋白质相互作用似乎正在发挥 在压抑机理中的作用。 除了Corepressor 浓度，这种寡聚可能作用于微调基因 规定。 已经使用荧光进行了几项研究 光谱法研究寡聚，但没有结构 迄今为止已经进行了研究。 NMR研究这种寡聚 会说出两个二聚体如何相互作用（即是否 四聚化是特定的或非特异性的，还是对称的 或不对称）。