NMR OF TRANSITION BETWEEN DIMER & TETRAMER OF EK18, MUTANT OF TRP REPRESSOR

二聚体之间转变的 NMR

• 批准号: 6309212
• 负责人: CATHERINE A ROYER
• 金额: $ 0.75万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-15 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6309212
• 关键词: biological products; biomaterials; biomedical resource; nuclear magnetic resonance spectroscopy; proteins

EK18 is a mutant of trp repressor and is cal a "super-repressor" since it represses the transcription of genes responsible for typtophan synthesis at a lower level of tryptophan molecules in the cell. Its molecular weight is about 12.5 kDaltons per monomer and it exists primarily as a dimer. However, when the salt concentration is lowered, or the protein concentration is increased, oligomerization occurs between dimers. The protein-protein interaction seems to play a role in the mechanism of repression. In addition to the corepressor concentration, this oligomerization may act to fine tune the gene regulation. Several studies have been done using fluorescence spectroscopy to investigate the oligomerization, but no structural studies have been done to date. NMR studies of this oligomerization will tell how the two dimers interact (i.e., whether the tetramerization is specific or nonspecific, or whether is it symmetric or unsymmetric).

EK18是trp抑制因子的突变体，被称为“超级抑制因子”。