SYNTHESIS OF LABELED CARBOHYDRATES

标记碳水化合物的合成

• 批准号: 6281487
• 负责人: LOUIS A SILKS
• 金额: $ 6.21万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-15 至 1999-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281487
• 关键词: biological products; biomedical resource; enzymes; nucleic acids

The structure and function studies of complex carbohydrates by nuclear magnetic resonance techniques has been hampered by the lack of both new methods for oligosaccharide assembly and appropriately stable isotope labeled precursors. Despite these limitations the body of experimentally derived 3D sugar structures is growing rapidly. With the advent of the Danishefsky solid phase synthetic method for construction of oligosaccharides (and other useful methods) we have embarked on a program to provide stable isotope labeled monomers for such methods and to investigate their use in solution NMR spectroscopic studies. The SIR has developed synthesis of the monomers, of D-[1,2,3,4,5,6- 13c 61glucal and D- [ 1,2,3,4,5,6- 13C 61galactal. from commercially available D-[U_13C]glucose. Live and co-workers have reported the first use of 13 C-labeled monomers in the Danishefsky solid phase method for the construction of a Lewisy determinant and have begun solution structure NMR studies on these labeled tetrasaccharides.

复合碳水化合物的结构和功能研究