SYNTHESIS OF LABELED CARBOHYDRATES

复合碳水化合物的结构和功能研究 核磁共振技术因缺乏 两种低聚糖组装的新方法和适当稳定 同位素标记的前体。尽管有这些限制，但 实验得出的3D糖结构正在迅速增长。使用 丹尼舍夫斯基固相合成法的问世 我们有低聚糖的结构(和其他有用的方法) 启动了一项计划，为以下物质提供稳定的同位素标记单体 这种方法及其在溶液核磁共振中的应用 光谱研究。SIR已经开发出一种合成 D-[1，2，3，4，5，6-13C 61葡萄糖醛和D-[1，2，3，4，5，6-13C]的单体 61个乳汁。从市售的D-[U_13C]葡萄糖中提取。现场直播 同事们报告说，13种碳标记单体首次在 Danishefsky固相法用于构造一个Lewis 并已开始对这些化合物进行溶液结构核磁共振研究 标记的四糖。

The structure and function studies of complex carbohydrates by nuclear magnetic resonance techniques has been hampered by the lack of both new methods for oligosaccharide assembly and appropriately stable isotope labeled precursors. Despite these limitations the body of experimentally derived 3D sugar structures is growing rapidly. With the advent of the Danishefsky solid phase synthetic method for construction of oligosaccharides (and other useful methods) we have embarked on a program to provide stable isotope labeled monomers for such methods and to investigate their use in solution NMR spectroscopic studies. The SIR has developed synthesis of the monomers, of D-[1,2,3,4,5,6- 13c 61glucal and D- [ 1,2,3,4,5,6- 13C 61galactal. from commercially available D-[U_13C]glucose. Live and co-workers have reported the first use of 13 C-labeled monomers in the Danishefsky solid phase method for the construction of a Lewisy determinant and have begun solution structure NMR studies on these labeled tetrasaccharides.