SYNTHESIS OF LABELED CARBOHYDRATES

标记碳水化合物的合成

• 批准号: 6309029
• 负责人: LOUIS A SILKS
• 金额: $ 2.74万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2002-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6309029
• 关键词: biological products; biomedical resource; enzymes; nucleic acids

The structure and function studies of complex carbohydrates by nuclear magnetic resonance techniques has been hampered by the lack of both new methods for oligosaccharide assembly and appropriately stable isotope labeled precursors. Despite these limitations the body of experimentally derived 3D sugar structures is growing rapidly. With the advent of the Danishefsky solid phase synthetic method for construction of oligosaccharides (and other useful methods) we have embarked on a program to provide stable isotope labeled monomers for such methods and to investigate their use in solution NMR spectroscopic studies. The SIR has developed synthesis of the monomers, of D-[1,2,3,4,5,6- 13c 61glucal and D- [ 1,2,3,4,5,6- 13C 61galactal. from commercially available D-[U_13C]glucose. Live and co-workers have reported the first use of 13 C-labeled monomers in the Danishefsky solid phase method for the construction of a Lewisy determinant and have begun solution structure NMR studies on these labeled tetrasaccharides.

复合碳水化合物的结构和功能研究 核磁共振技术由于缺乏 这两种用于寡糖组装的新方法和适当稳定的 同位素标记的前体。 尽管有这些限制， 实验衍生的3D糖结构正在迅速增长。 与 Danishefsky固相合成法的出现， 构建寡糖（和其他有用的方法），我们有 开始了一项计划，提供稳定的同位素标记单体， 这些方法，并探讨其在溶液NMR中的应用 光谱研究 SIR已经开发了合成 D-[1，2，3，4，5，6 - 13 c] 61葡萄糖醛和D- [1，2，3，4，5，6 - 13 c] 61半乳糖醛。 由市售的D-[U_13C]葡萄糖制备。 生活和 同事报道了13 C标记的单体首次用于 Danishefsky固相法构造Lewisy方程 决定因素，并已开始对这些溶液结构核磁共振研究 标记的四糖。