DNA BIOSENSOR ARRAY FOR SINGLE CELL GENOMICS ANALYSIS

用于单细胞基因组分析的 DNA 生物传感器阵列

• 批准号: 6188464
• 负责人: JOSEPH WANG
• 金额: $ 17.27万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-28 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188464
• 关键词: DNA; bioengineering /biomedical engineering; biomedical equipment development; biosensor device; electrochemistry; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; single cell analysis

The goal of this collaborative project is to develop an automated hand-held micromachined electrochemical flow analyzer for high-speed single-cell genomics analysis. Innovative biosensor technology, based on new highly selective and sensitive PNA-dendrimer recognition chemistry, high-density parallel-channel hybridization microreactors, a label-free electrochemical detection of the released targets at downstream microelectrodes, and state-of-the-art microfabrication/micromachining technology will be used for meeting the challenges of rapid and sensitive single-cell nucleic-acid sequencing.

该合作项目的目标是开发一种自动化手持式微机械电化学流分析仪，用于高速单细胞基因组学分析。 基于新型高选择性和灵敏的 PNA 树枝状大分子识别化学、高密度并行通道杂交微反应器、下游微电极释放靶标的无标记电化学检测以及最先进的微加工/微加工技术的创新生物传感器技术将用于应对快速灵敏的单细胞核酸的挑战 测序。

项目成果

Stripping analysis of nucleic acids at a heated carbon paste electrode.

• DOI: 10.1021/ac000286q
• 发表时间: 2000-07
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Joseph Wang;P. Gründler;G. Flechsig;M. Jasinski;G. Rivas;E. Sahlin;J. L. L. Paz-J.-L.-L.-Paz-47599710

On-demand electrochemical release of DNA from gold surfaces.

从金表面按需电化学释放 DNA。

• DOI: 10.1016/s0302-4598(00)00081-7
• 发表时间: 2000
• 期刊: Bioelectrochemistry (Amsterdam, Netherlands)
• 作者: Wang,J;Jiang,M;Mukherjee,B
• 通讯作者: Mukherjee,B