DEVELOPMENT OF BIOSELECTIVE VOLTAMMETRIC SENSORS

本研究的目标是发明、设计、研究和测试 适合临床使用的高效伏安型生物传感器 治疗性监测。主要战略将继续是 分析物优先堆积到工作台上 电极表面，然后伏安定量 表面结合的物种。分析物预浓缩将是 通过选择性连接，通过共价或离子- 修饰电极上的交换反应，或优先反应 在普通电极上的吸附。聚合体涂料，带 可控制的渗透率，将被评估为改善 传感器的选择性和稳定性。一种适用于 各种具有临床意义的分析物，特别是治疗性的 毒品，将被调查。区别于 建议的传感器具有固有的敏感度( 预富集步骤和高级伏安技术)， 选择性(基于定义的化学反应和 聚合物涂层的区别性质)，简单性， 经济、快速和小型化。这项调查将包括 这种生物传感器的基本和实用两个方面， 包括对传感器行为的详细研究，系统性 优化经营条件，竞争和 对所产生的方案进行临床测试。待解决的具体问题 解决的问题包括高灵敏的吸附反萃 抗癌药物和微量元素检测方案， 对电极进行适当的剪裁(修改)以确保有效 用作预聚焦面，因以下原因产生的干涉 内源性生物材料、联合用药或 主要代谢物，新的修饰电极基于 复合材料和双层聚合物组件和混合碳 浆料、表面条件和电极处理的影响 吸附溶出响应的程序和重现性 重新生成新的传感器表面。通过适当地处理 上述问题将获得显著的分析优势， 伏安型生物传感器在临床上的应用 工具将更接近于实现。

The goal of this research is to invent, design, study and test effective voltammetric biosensors suitable for use in clinical therapeutic monitoring. The principal strategy will continue to be the preferential accumulation of the analyte onto the working electrode surface, followed by voltammetric quantitation of the surface-bound species. Analyte preconcentration will be accomplished by selective attachment, via covalent or ion- exchange reactions at modified electrodes, or preferential adsorption at ordinary electrodes. Polymeric coatings, with controllable permeability, will be evaluated for improving the selectivity and stability of the sensors. Sensing schemes for a variety of clinically-significant analytes, particularly therapeutic drugs, will be investigated. The features that distinguish the proposed sensors are inherent sensitivity (combination of a preconcentration step and advanced voltammetric techniques), selectivity (based on defined chemical reactions and discriminative properties of polymeric coatings), simplicity, economy, speed, and miniaturization. The investigation will cover both the fundamental and practical aspects of such biosensors, including detailed studies of the sensor behavior, systematic optimization of operational conditions, and competition and clinical tests of the resulting schemes. Specific issues to be addressed include highly sensitive adsorptive stripping measurement schemes for anticancer drugs and trace elements, appropriate tailoring (modification) of electrodes for effective use as preconcentrating surfaces, interferences due to endogeneous biological materials, coadministrated drugs, or principal metabolites, new modified electrodes based on composite and bilayer polymer assemblies and mixed carbon paste, effects of surface conditions and electrode treatment procedures on the adsorptive stripping response, and reproducible regeneration of a fresh sensor surface. By properly addressing the above issues significant analytical advantages will be achieved, and the utility of voltammetric biosensors as practical clinical tools will come much closer to realization.