DEVELOPMENT OF BIOSELECTIVE VOLTAMMETRIC SENSORS

不同的生物选择性伏安电极将被开发为 用于药物和临床重要化合物的有效传感器 体液。通过以下方式实现更高的选择性和灵敏度 所需化合物(或化合物组)的受控积累 放在电极表面上。这一预浓缩步骤将是 通过选择性连接(通过共价反应)或 优先吸附在修饰电极或普通电极表面。 电极覆盖，用适当的膜或聚合物，将提供 通过控制对表面的访问来增加选择性。跟随 受控的预富集步骤，电极将被转移 从样品溶液到合适的空白溶液，其中 表面结合的物种将被量化。这种“媒介交换”程序 将通过消除由于以下原因引起的背景电流来增强选择性 溶液相物种。大量的药物，维生素，有毒的有机 化合物和其他临床上重要的化合物将被测试为 这种生物选择性伏安检测的候选者。为了 实现对每个分析物的最佳响应(高特异性和 最小矩阵干扰)，我们将检查各种实验 条件，如电极材料和功能，解决方案 组成、pH、温度、传质或膜孔隙率。五花八门 将检查表面改性程序以获得特定的 回应。这些操作条件的最佳组合将是 用于操作所设计的浸入式临床传感器。 竞赛实验将被设计用来测试 传感器。应用于各种药物的选择性测量， 使用间歇和流动系统测定体液中的生化物质和致癌物质 将进行测试和演示。

Different bioselective voltammetric electrodes will be developed as effective sensors for pharmaceutical and clinically important compounds in body fluids. Enhanced selectivity and sensitivity will be achieved by controlled accumulation of the desired compound (or group of compounds) onto the electrode surface. This preconcentration step will be accomplished by selective attachment (via covalent reaction) or preferential adsorption to surfaces of modified or ordinary electrodes. Electrode coverage, with a proper membrane or polymer, would provide additional selectivity by controlling the access to the surface. Following the controlled preconcentration step, the electrode will be transferred from the sample solution to a suitable blank solution where the surface-bound species will be quantified. Such 'medium exchange' procedure will enhance the selectivity by eliminating background currents due to solution-phase species. Large array of drugs, vitamins, toxic organic compounds and other compounds of clinical importance will be tested as candidates for this bioselective voltammetric detection. In order to realize the best possible response for each analyte (high specificity and minimum matrix interferences), we will examine various experimental conditions, such as electrode material and functionalities, solution composition, pH, temperature, mass-transport or membrane porosity. Various surface modification procedures will be examined for obtaining the specific response. The optimum combination of these operating conditions will be used in the operation of the designed dipping-type clinical sensors. Competition experiments will be designed to test the specificity of the sensor. Applications to selective measurements of various drugs, biochemical and carcinogens in body fluids, using batch and flow systems will be tested and demonstrated.