CORE--QUANTITATIVE MOLECULAR ANALYSIS

核心--定量分子分析

• 批准号: 6203477
• 负责人: Gary J Nabel
• 金额: $ 17.33万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203477
• 关键词: AIDS; analytical chemistry; biomedical facility; fluorescent dye /probe; gene expression; nucleic acid quantitation /detection; nucleic acid sequence; polymerase chain reaction

The QUANTITATIVE MOLECULAR ANALYSIS CORE will support novel, innovate state-of-the-art fluorescence detection and kinetic PCR technology using the ABI 7700 Sequence Detector machine to sensitively, specifically and reproducibly quantify target DNA and RNA sequences in blood and tissue with high through put. Thus, making it possible for them to readily quantify a range of genes and gene transcripts applicable to their particular area of research. Additionally, the core will provide for the construction of stringency matched primers for target sequence amplification and for the construction of sequence-specific fluorescence- linked oligonucleotides for target sequence detection. The quantitative molecular analysis core will be co-directed by Drs. Nabel and Wolinsky of the University of Michigan and Northwestern University, respectively. Dr. Nabel and Dr. Betty Wu, his staff member at the University of Michigan, and Dr. Wolinsky and Dr. Clark Brown and Ms. Patricia Otto, his staff at Northwestern University, will be responsible for overseeing, in detail, the specimen handling, DNA and RNA processing and oligonucleotide primer and probe sequence design and construction that conforms to the strict stringency requirements of the assay. The goal of the DNA sequencing core is to: (1) provide a core laboratory of high through put, reproducible quantitative DNA and RNA analysis; (2) provide for the construction of stringency matched primers for target sequence amplification; and (3) provide for the construction of sequence-specific fluorescence-linked oligonucleotides for target sequence detection. The quantitative molecular analysis core facilitates interactions among researchers, allows researchers to readily access automated quantitative molecular analysis, lowers the cost of this technology to individual researchers, and facilitates the undertaking of new projects.

定量分子分析核心将支持新颖、创新 采用最先进的荧光检测和动态 PCR 技术 ABI 7700 序列检测器机器能够灵敏、特异性和 可重复地量化血液和组织中的目标 DNA 和 RNA 序列 具有高吞吐量。因此，使他们能够轻松地 量化一系列适用于其的基因和基因转录本 特定的研究领域。此外，该核心还将提供 构建靶序列严格匹配的引物 扩增和构建序列特异性荧光- 用于靶序列检测的连接寡核苷酸。定量 分子分析核心将由博士共同指导。纳贝尔和沃林斯基 分别是密歇根大学和西北大学。博士。 纳贝尔和他在密歇根大学的工作人员 Betty Wu 博士， 以及沃林斯基博士、克拉克·布朗博士和帕特里夏·奥托女士，以及他在 西北大学将负责详细监督， 样本处理、DNA 和 RNA 处理以及寡核苷酸引物 以及符合严格要求的探针序列设计和构建 测定的严格要求。 DNA测序核心的目标 目的是：（1）提供高通量、可重复的核心实验室 定量 DNA 和 RNA 分析； (2) 规定建设 用于靶序列扩增的严格匹配引物；和（3） 提供序列特异性荧光连接的构建 用于靶序列检测的寡核苷酸。定量分子 分析核心促进研究人员之间的互动，允许 研究人员可以轻松进行自动化定量分子分析， 降低了个体研究人员使用该技术的成本，并且 有利于新项目的开展。