CORE--HISTOTECHNOLOGY LABORATORY

核心--组织技术实验室

• 批准号: 6299905
• 负责人: Henry C Pitot, Jr.
• 金额: $ 21.64万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299905
• 关键词: autoradiography; biomedical facility; chemical carcinogenesis; cryostat; histochemistry /cytochemistry; histopathology; tissue /cell preparation

The Histotechnology Laboratory, under the supervision of Dr. Henry C. Pitot is located in four modules (approximately 88 square feet of space in the basement of the McArdle Laboratory. A total of five research specialists and one laboratory technician work full or part-time in the facility as does a half-time student laboratory assistant. The Laboratory is equipped with two chemical fume hoods, two cytostats, two automated tissue processors, four Leitz microtomes, Tissue Tek Embedding Center, microtome knife sharpener, Pope Solvent Recovery System, a freezer, refrigerator, and other small equipment necessary for the operation of the facility. The Histotechnology Laboratory is equipped to perform a wide range of services including the preparation of histological sections from both paraffin-embedded and cryostat material, histochemical staining (e.g., gamma-glutamyltranspeptidase, glucose-6-phosphatase, ATPase), special stains (such as PAS, Giemsa, Iron) and special procedures (autoradiography, immunohistochemistry, block preparations of cell pellets or embryoid bodies, in situ staining, and histochemistry and autoradiography of cell cultures.

在亨利博士的监督下，组织技术实验室。 皮托管位于四个模块（约88平方英尺的空间， 麦卡德尔实验室的地下室共5项研究 专家和一名实验室技术员全职或兼职工作， 实验室助理半职学生。实验室 配备了两个化学通风柜，两个细胞恒温器，两个自动 组织处理器，四台Leitz切片机，Tissue Tek Embedding Center， 切片机刀片锋利，波普溶剂回收系统，冷冻机， 冰箱和其他小型设备的运作所需的 设施。组织技术实验室配备了执行广泛的 一系列服务，包括组织切片的制备， 石蜡包埋和冷冻材料，组织化学染色 (e.g.,γ-谷氨酰转肽酶，葡萄糖-6-磷酸酶，ATP酶）， 特殊染色（如PAS、Giemsa、铁）和特殊程序 （放射自显影，免疫组织化学，细胞团块制备 或胚状体，原位染色和组织化学， 细胞培养物的放射自显影。