CORE--MICROSCOPY FACILITY

核心——显微镜设备

• 批准号: 6299903
• 负责人: Henry C Pitot, Jr.
• 金额: $ 21.64万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299903
• 关键词: bioimaging /biomedical imaging; biomedical facility; electron microscopy; light microscopy

The McArdle Electron Microscopy Facility has been supported in part by the CCSG for over 30 years. For the next grant period we are consolidating several resources related to light microscopy with this facility (renamed the Microscopy Facility) to improve their upkeep and facilitate training of students and fellows in their use. This is appropriate since the expertise needed for electron microscopy and its interpretation overlaps with that for light microscopy. The two goals of the Electron Microscopy component, which is located in a 300 square foot laboratory basement of McArdle, are: (1) to provide basic electron microscopy services to all research groups in the McArdle Laboratory; (2) to perform collaborative studies with principle investigators and their graduate students and postdoctoral fellows. Having a self sufficient transmission microscopy facility located in the McArdle Laboratory (in the basement) permits easy access to all faculty members and is essential to instructing graduate students in the basic principles of electron microscopy. The facility contains a Hitachi H-7000 electron microscope, a new Reichart Ultracut E and two Sorvall MT-2 ultramicrotomes, a Denton vacuum evaporator, and darkroom facilities for developing negatives and making prints with a state-of-the-art print processor. Facilities for visible light and fluorescence microscopy are located in a 200 square foot laboratory on the 3rd floor of McArdle. The instrumentation includes a Zeiss Axiophot which is capable of a variety of different types of light microscopy as well as photomicroscopy. In addition, a Leitz research microscope with fluorescence and photomicrographic capabilities is available in the unit for general use. Images may be documented through the use of standard film cameras attached to the microscope or a CCD CAMERA, allowing later computer analysis of digital images.

麦卡德尔电子显微镜设施得到了部分支持， CCSG超过30年。在下一个资助期，我们将 一些与光学显微镜相关的资源（已更名为 显微镜设施），以改善其保养和促进培训 学生和研究员在他们的使用。这是适当的，因为 电子显微镜所需的专业知识及其解释重叠 用于光学显微镜检查。 电子显微镜组件的两个目标，它位于一个 300平方英尺的McArdle实验室地下室，主要有：（1）提供基本的 为麦卡德尔的所有研究小组提供电子显微镜服务 实验室;（2）进行原则性的合作研究 研究人员和他们的研究生和博士后研究员。具有 位于麦卡德尔的自给自足的透射显微镜设施 实验室（在地下室）允许方便地访问所有教职员工 这对指导研究生掌握基本原理至关重要 电子显微镜。该设施包含一台日立H-7000电子 显微镜，一个新的Reichart Ultracut E和两个Sorvall MT-2 超薄切片机，丹顿真空蒸发器，和暗室设施， 冲洗底片，用最先进的照片制作照片 处理器. 可见光和荧光显微镜的设施位于 200平方英尺的实验室在麦卡德尔的三楼。的 仪器包括蔡司Axiophot，其能够进行各种 不同类型的光学显微镜以及显微摄影。在 此外，Leitz研究显微镜与荧光和 该装置具有显微照相功能，可供一般使用。 图像可以通过使用标准胶片相机记录下来 到显微镜或CCD相机，允许稍后的计算机分析， 数字图像。