CORE--MICROSCOPY FACILITY

核心——显微镜设备

• 批准号: 6299903
• 负责人: Henry C Pitot, Jr.
• 金额: $ 21.64万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299903
• 关键词: bioimaging /biomedical imaging; biomedical facility; electron microscopy; light microscopy

The McArdle Electron Microscopy Facility has been supported in part by the CCSG for over 30 years. For the next grant period we are consolidating several resources related to light microscopy with this facility (renamed the Microscopy Facility) to improve their upkeep and facilitate training of students and fellows in their use. This is appropriate since the expertise needed for electron microscopy and its interpretation overlaps with that for light microscopy. The two goals of the Electron Microscopy component, which is located in a 300 square foot laboratory basement of McArdle, are: (1) to provide basic electron microscopy services to all research groups in the McArdle Laboratory; (2) to perform collaborative studies with principle investigators and their graduate students and postdoctoral fellows. Having a self sufficient transmission microscopy facility located in the McArdle Laboratory (in the basement) permits easy access to all faculty members and is essential to instructing graduate students in the basic principles of electron microscopy. The facility contains a Hitachi H-7000 electron microscope, a new Reichart Ultracut E and two Sorvall MT-2 ultramicrotomes, a Denton vacuum evaporator, and darkroom facilities for developing negatives and making prints with a state-of-the-art print processor. Facilities for visible light and fluorescence microscopy are located in a 200 square foot laboratory on the 3rd floor of McArdle. The instrumentation includes a Zeiss Axiophot which is capable of a variety of different types of light microscopy as well as photomicroscopy. In addition, a Leitz research microscope with fluorescence and photomicrographic capabilities is available in the unit for general use. Images may be documented through the use of standard film cameras attached to the microscope or a CCD CAMERA, allowing later computer analysis of digital images.

McArdle电子显微镜设备得到了 CCSG已有30多年的历史。对于下一个授权期，我们正在巩固 与该设备的光学显微镜相关的几个资源(已重命名 显微镜设备)，以改进他们的维护和促进培训 学生和研究员的使用情况。这是适当的，因为 电子显微镜及其解释所需的专业知识重叠 用来做光学显微镜。 电子显微镜组件的两个目标是位于一个 300平方英尺的McArdle实验室地下室，有：(1)提供基本 电子显微镜为McArdle的所有研究小组提供服务 实验室；(2)有原则地开展合作研究 调查人员及其研究生和博士后研究员。拥有 位于McArdle的自给自足的透射式显微镜设备 实验室(在地下室)允许所有教职员工轻松进入 对于教授研究生基本原则是必不可少的。 电子显微镜。该设施包含一个日立H-7000电子设备 显微镜，一种新的Reichart Ultraut E和两台Sorvall MT-2 超微显微镜、Denton真空蒸发器和暗室设施 用最先进的印刷品冲洗底片和制作印刷品 处理器。 可见光和荧光显微镜的设施位于 200平方英尺的实验室位于McArdle的3楼。这个 仪器设备包括蔡司Axiophot，它能够进行各种 不同类型的光学显微镜以及光学显微镜。在……里面 此外，莱茨研究显微镜具有荧光和 该设备具有显微照相功能，可供一般使用。 图像可以通过使用所附的标准胶片相机来记录 到显微镜或ccd摄像机，允许以后的计算机分析 数字图像。