NMR INVESTIGATIONS OF BIOMOLECULAR STRUCTURE OF SRY COMPLEXES IN SOLUTION

溶液中 SRY 复合物生物分子结构的 NMR 研究

• 批准号: 6309186
• 负责人: MICHAEL Aaron WEISS
• 金额: $ 0.75万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-15 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6309186
• 关键词: biological products; biomaterials; biomedical resource; nuclear magnetic resonance spectroscopy; nucleic acids; structural biology

A number of human and mouse native as well as mutant SRY complexes with duplex DNA of various length and composition are being studied in our laboratory. Doubly labeled protein complexed with DNA is subjected to a series of 2D or 3D heteronuclear and triple resonance experiments in order to provide assignments and eventually a large number of identifiable NOE constraints for 3D structure calculations. We have developed a set of triple resonance experiments optimized for larger molecules and using non-compromising shaped, selective pulses and decoupler pulse trains that in theory should give significant improvement over any of the conventional triple resonance experiments as described in the current literature. We propose to compare results obtained with the different sets of sequences on our 600 and 600 MHz spectrometers with some data to be obtained on 600 and 500 MHz instruments at the NMRFAM facility.

许多人和小鼠的天然以及突变的SRY复合物 不同长度和组成的双链DNA正在研究中， 我们的实验室 与DNA复合的双标记蛋白质， 经受一系列2D或3D异频和三重共振 实验，以提供作业，并最终一个大的 用于三维结构计算的可识别NOE约束的数量。 我们已经开发了一套三重共振实验优化， 更大的分子，并使用非妥协的形状，选择性脉冲 和倍频脉冲序列，理论上应该给显着的 对任何常规三重共振实验的改进 如现有文献中所述。 我们建议比较结果 在我们的600和600 MHz上使用不同的序列集获得 在600和500 MHz上获得某些数据的光谱仪 NMRFAM设施的仪器。