OPERATION OF AN INFRARED BEAMLINE DEDICATED TO STUDY OF BIO MEDICAL PROBLEMS

作为研究软骨下骨发育的模型，我们 正在检查骨质疏松症小鼠(OP/OP小鼠)的关节。 在骨质疏松症中，破骨细胞形成减少会阻止骨骼 吸收，导致骨骼的净过度生长。操作/操作鼠标是 由于脑脊液-1(集落刺激)的改变而导致的骨腐症 因子-1)基因。这种突变，即胸苷262碱基的插入 在CSF-1基因中ATG起始密码子下游的对，导致 移帧和引入提前终止密码子。作为一名 结果，突变的基因编码一个截短的蛋白质产物，约 60个氨基酸，缺乏生物活性。正常脑脊液-1 刺激破骨细胞前体细胞分化为 破骨细胞，也可能调节成熟的破骨细胞。在业务/业务中 小鼠缺乏脑脊液-1可导致骨化症 破骨细胞数量的减少；产生的骨骼不能被吸收。 斯坦利博士实验室和其他地方的研究表明， 自发性衰老或OP内注射重组人CSF-1 小鼠从出生3天起，就有能力修复骨干骨 (即股骨骨干中的骨)到更正常的组织学，但 软骨下骨仍为岩质骨。哈默曼博士和斯坦利博士将 检查巨噬细胞和破骨细胞随时间的变化 衰老和csf-1处理的小鼠的软骨下和骨干骨。在……里面 此外，上覆关节软骨的组织学状态 将进行检查以确定岩性软骨下骨是否 与上覆关节软骨的退变有关。

As a model for examining the development of subchondral bone, we are examining the joints of mice who are osteopetrotic (op/op mice). In osteoporosis, reduced osteoclast formation prevents bone resorption, leading to a net overgrowth of bone. The op/op mouse is osteopetrotic due to an alteration in the CSF-1 (Colony Stimulating Factor-1) gene. This mutation, the insertion of a thymidine 262 base pairs downstream of the ATG start codon in the CSF-1 gene, leads to a frame shift and the introduction of a premature stop codon. As a result, the mutated gene encodes a truncated protein product of about 60 amino acids, which lacks biological activity. CSF-1 normally stimulates the differentiation of osteoclast progenitors into osteoclasts and may also regulate mature osteoclasts. In the op/op mouse, the lack of CSF-1 leads to osteopetrosis due to a marked reduction in osteoclast numbers; the bone produced cannot be resorbed. Studies in Dr. Stanley's lab and elsewhere have shown that either spontaneous aging or injections of human recombinant CSF-1 into op mice from 3 days of life, is capable of reverting diaphyseal bone (i.e. bone in the shaft of the femur) to more normal histology, but subchondral bone remains petrotic. Drs. Hamerman and Stanley will examine the macrophage and osteoclast populations over time in the subchondral and diaphyseal bone in aging and CSF-1 treated mice. In addition, the histologic state of the overlying articular cartilage will be examined to determine if petrotic subchondral bone is associated with degeneration of the overlying articular cartilage.