OPERATION OF AN INFRARED BEAMLINE DEDICATED TO STUDY OF BIO MEDICAL PROBLEMS
OPERATION OF AN INFRARED BEAMLINE DEDICATED TO STUDY OF BIO MEDICAL PROBLEMS
批准号:
6345096
负责人:
MARK R CHANCE
金额:
$6.52万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-09-01 至 2001-08-31
中文摘要
作为检查软骨下骨发育的模型,我们
正在检查患骨硬化症的小鼠(OP/OP小鼠)的关节。
在骨质疏松症中,破骨细胞形成减少,
吸收,导致骨的净过度生长。 OP/OP鼠标
由于CSF-1(集落刺激因子)的改变,
因子-1)基因。 这种突变,插入一个胸苷262碱基
在CSF-1基因中的ATG起始密码子下游对,导致
移码和提前终止密码子的引入。 作为
结果,突变的基因编码截短的蛋白质产物,约
60个氨基酸,缺乏生物活性。 CSF-1正常
刺激破骨细胞祖细胞分化为
破骨细胞,也可以调节成熟的破骨细胞。 在OP/OP
在小鼠中,CSF-1的缺乏导致骨硬化症,这是由于
破骨细胞数量减少;产生的骨不能被吸收。
斯坦利博士实验室和其他地方的研究表明,
自发老化或将人重组CSF-1注射入OP
小鼠从出生3天起,能够恢复骨干骨
(i.e.股骨干中的骨)到更正常的组织学,但是
软骨下骨仍为岩质。 哈尔曼医生和斯坦利医生
检查巨噬细胞和破骨细胞群体随着时间的推移,
老化和CSF-1处理的小鼠中的软骨下骨和骨干骨。 在
此外,上覆关节软骨的组织学状态
将进行检查,以确定岩质软骨下骨是否
与上覆关节软骨的退化有关。
英文摘要
As a model for examining the development of subchondral bone, we
are examining the joints of mice who are osteopetrotic (op/op mice).
In osteoporosis, reduced osteoclast formation prevents bone
resorption, leading to a net overgrowth of bone. The op/op mouse is
osteopetrotic due to an alteration in the CSF-1 (Colony Stimulating
Factor-1) gene. This mutation, the insertion of a thymidine 262 base
pairs downstream of the ATG start codon in the CSF-1 gene, leads to a
frame shift and the introduction of a premature stop codon. As a
result, the mutated gene encodes a truncated protein product of about
60 amino acids, which lacks biological activity. CSF-1 normally
stimulates the differentiation of osteoclast progenitors into
osteoclasts and may also regulate mature osteoclasts. In the op/op
mouse, the lack of CSF-1 leads to osteopetrosis due to a marked
reduction in osteoclast numbers; the bone produced cannot be resorbed.
Studies in Dr. Stanley's lab and elsewhere have shown that either
spontaneous aging or injections of human recombinant CSF-1 into op
mice from 3 days of life, is capable of reverting diaphyseal bone
(i.e. bone in the shaft of the femur) to more normal histology, but
subchondral bone remains petrotic. Drs. Hamerman and Stanley will
examine the macrophage and osteoclast populations over time in the
subchondral and diaphyseal bone in aging and CSF-1 treated mice. In
addition, the histologic state of the overlying articular cartilage
will be examined to determine if petrotic subchondral bone is
associated with degeneration of the overlying articular cartilage.
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Research Support Core C: Computational Biology in Substance Use
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批准号:10304585
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项目类别:
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资助金额:$70.74万
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财政年份:2021
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负责人:MARK R CHANCE
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依托单位:
Research Support Core C: Computational Biology in Substance Use
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批准号:10632097
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项目类别:
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资助金额:$73.51万
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财政年份:2021
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负责人:MARK R CHANCE
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依托单位:
PhosphoExplorer: Software for annotating and analyzing phosphoproteomics data
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批准号:9176205
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项目类别:
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资助金额:$36.02万
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财政年份:2016
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负责人:MARK R CHANCE
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依托单位:
PhosphoExplorer: Software for annotating and analyzing phosphoproteomics data
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批准号:9339710
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项目类别:
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资助金额:$36.02万
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财政年份:2016
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负责人:MARK R CHANCE
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依托单位:
PhosphoExplorer: Software for annotating and analyzing phosphoproteomics data
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批准号:9518976
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项目类别:
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资助金额:$36.02万
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财政年份:2016
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负责人:MARK R CHANCE
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依托单位:
STRUCTURAL AND FUNCTIONAL WATER DYNAMICS IN RHODOPSIN ACTIVATION FROM PICOSECON
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批准号:8364358
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项目类别:
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资助金额:$0.11万
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财政年份:2011
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负责人:MARK R CHANCE
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依托单位:
Structure, Dynamics & Activation Mechanisms of Chemokine Receptors
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批准号:8153254
-
项目类别:
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资助金额:$12.09万
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财政年份:2010
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负责人:MARK R CHANCE
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依托单位:
Radiolytic Footprinting Methods for Structural Mass Spectrometry
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批准号:8265984
-
项目类别:
-
资助金额:$27.16万
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财政年份:2010
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负责人:MARK R CHANCE
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依托单位:
CWRU Short-Term Heart Lung and Blood Research Opportunities Program
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批准号:8277375
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项目类别:
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资助金额:$16.21万
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财政年份:2010
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负责人:MARK R CHANCE
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依托单位:
Radiolytic Footprinting Methods for Structural Mass Spectrometry
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批准号:8070036
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项目类别:
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资助金额:$27.16万
-
财政年份:2010
-
负责人:MARK R CHANCE
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依托单位:
Radiolytic Footprinting Methods for Structural Mass Spectrometry
-
批准号:8440819
-
项目类别:
-
资助金额:$25.62万
-
财政年份:2010
-
负责人:MARK R CHANCE
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依托单位:
Radiolytic Footprinting Methods for Structural Mass Spectrometry
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批准号:7896398
-
项目类别:
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资助金额:$28.26万
-
财政年份:2010
-
负责人:MARK R CHANCE
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依托单位:
Thermo Electron LTQ Orbitrap XL ETD with ESI and Dionex Ultimate 3000 HPLC System
-
批准号:7838746
-
项目类别:
-
资助金额:$98.3万
-
财政年份:2010
-
负责人:MARK R CHANCE
-
依托单位:
Case Proteomics Center for HIV/AIDS and Drug Abuse
-
批准号:7847942
-
项目类别:
-
资助金额:$2.17万
-
财政年份:2009
-
负责人:MARK R CHANCE
-
依托单位:
Case Center for Synchrotron Bioscience
-
批准号:8740761
-
项目类别:
-
资助金额:$99.88万
-
财政年份:2009
-
负责人:MARK R CHANCE
-
依托单位:
Case Center for Synchrotron Bioscience
-
批准号:8936209
-
项目类别:
-
资助金额:$3.78万
-
财政年份:2009
-
负责人:MARK R CHANCE
-
依托单位:
Case Proteomics Center for HIV/AIDS and Drug Abuse
-
批准号:8053331
-
项目类别:
-
资助金额:$97.41万
-
财政年份:2009
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负责人:MARK R CHANCE
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依托单位:
Case Proteomics Center for HIV/AIDS and Drug Abuse
-
批准号:7805490
-
项目类别:
-
资助金额:$99.44万
-
财政年份:2009
-
负责人:MARK R CHANCE
-
依托单位:
Case Center for Synchrotron Bioscience
-
批准号:9127952
-
项目类别:
-
资助金额:$82.6万
-
财政年份:2009
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负责人:MARK R CHANCE
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依托单位:
Case Center for Synchrotron Biosciences
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批准号:8337422
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项目类别:
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资助金额:$6.64万
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财政年份:2009
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负责人:MARK R CHANCE
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依托单位:
海外基金