OPERATION OF BEAMLINE X 9A RESOURCE DEDICATED TO TIME RESOLVED XRAY FOOTPRINTING

专用于时间分辨 X 射线足迹的 BEAMLINE X 9A 资源的操作

• 批准号: 6345073
• 负责人: MARK R CHANCE
• 金额: $ 5.38万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345073
• 关键词: biomedical equipment development; biomedical resource; biotechnology; building /facility design /renovation; nucleic acids; proteins; radiology; spectrometry; technology /technique

To provide dedicated access to footprinting technology, beamline X9A at the National Synchrotron Light Source is maintained along with a dedicated stopped flow instrument to allow footprinting studies for our expanded group of investigators. The beamline accepts up to 5 milliradians of white beam, with a flux density of 109 photons/s/mm2/0.07% bandwidth over an effective energy range of 3-30 keV. A dedicated beamline is necessary for high productivity of the core and collaborative research project. Our Resource is the world leader in this technology and the availability of a dedicated beamline will continue our leadership in this area. We are now routinely conducting footprinting studies on 5-10 msec and longer timescales with our stopped flow device. Major advances in understanding RNA folding have been accomplished and studies of protein-DNA complex formation have been initiated. The dedicated footprinting beamline will be moved to a new station (X28C) in late 1999 to make way for the new crystallography program at X9A. The resource has been given access to the new beamline by the NSLS.

为了提供足迹技术的专用通道， 国家同步加速器光源的X9 A沿着与 一个专用的停流仪器，允许足迹研究， 我们扩大的调查小组 光束线接受高达5 毫弧度的白色光束，通量密度为109 光子/s/mm 2/0.07%带宽，有效能量范围为3-30 凯 专用的光束线对于激光器的高生产率是必要的。 核心和合作研究项目。 我们的资源是世界 该技术的领先者，并提供专用光束线 我们将继续在这一领域发挥领导作用。 我们现在经常 在5-10毫秒和更长的时间尺度上进行足迹研究 我们的断流装置 理解RNA的重大进展 蛋白质-DNA复合物的研究 组建已经启动。 专用足迹光束线 将于一九九九年年底迁往新车站（X28 C）， 新的晶体学项目 资源已被赋予 新光束线的使用权