TRANSIENT INFRARED SPECTROSCOPY

瞬态红外光谱

• 批准号: 6328023
• 负责人: ROBIN Main HOCHSTRASSER
• 金额: $ 2.18万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6328023
• 关键词: biomaterials; biomedical resource; proteins

Infrared spectroscopy allows access to the vibrational levels of molecules and thus permits the study of intermediates formed during optically triggered events. This research concerns the development of high repetition rate infrared spectroscopy aimed at studying protein dynamics via the vibrational spectra of the intermediate states. The thrust of this effort is the design of an optical parametric generator/amplifier system using silver thiogallate and gallium selenide crystals. This design, which is now being implemented, is enabling the acquisition of transient infrared spectra over the range 500 to 3000 cm-1. The system is using KTP and BBO OPA's to fill in the region from 3000 to 10,000 cm-1. This laser system is capable of generating extremely short pulses of visible (20fs) and infrared (40fs) radiation. In addition, we have coupled a 32 element MCT multiplexed detector array into the infrared probe generating system. With this system we have the capability of obtaining spectral measurements of coherent infrared signals. This allows for spectral resolution of infrared photon echoes, providing detailed information of vibrational energy flow within peptide and protein moieties. In total, we are developing pump/probe, transient grating, photon echo and heterodyned photon echo methods as part of the thrust in ultrafast infrared spectroscopy for proteins.

红外光谱可以获取振动水平 分子，从而允许研究过程中形成的中间体 光学触发事件。 这项研究涉及开发 用于研究蛋白质的高重复率红外光谱 通过中间态的振动谱进行动力学。 这 这项工作的主旨是设计一个光学参数 使用硫代镓酸银和镓的发生器/放大器系统 硒化物晶体。 目前正在实施的这一设计是 能够采集整个范围内的瞬态红外光谱 500 至 3000 cm-1。 系统使用KTP和BBO OPA来填写 范围从 3000 到 10,000 cm-1。 该激光系统能够 产生极短的可见光 (20fs) 和红外脉冲 （40fs）辐射。 此外，我们还耦合了 32 元件 MCT 多路复用探测器阵列进入红外探头生成系统。 有了这个系统，我们就有能力获得光谱 相干红外信号的测量。 这允许光谱 红外光子回波分辨率，提供详细信息 肽和蛋白质部分内的振动能量流。 在 总的来说，我们正在开发泵浦/探针、瞬态光栅、光子回波 和外差光子回波方法作为超快推力的一部分 蛋白质的红外光谱。