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HUMAN CHROMOSOME ANALYSIS & SORTING

HUMAN CHROMOSOME ANALYSIS & SORTING
人类染色体分析
批准号：
6327951
负责人：
SCOTT CRAM
金额：
$0.31万
依托单位：
UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
项目状态：
已结题
起止时间：
2000-07-01 至 2001-06-30
来源：
https://reporter.nih.gov/project-details/6327951
关键词：
biomedical resource biotechnology genetics genome human tissue technology /technique

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中文摘要
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英文摘要
The human genome program has been facilitated, in part, by the ability to sort human chromosomes. Up to 2 5g of each of the 24 human chromosome types are being sorted for construction of chromosome-specific DNA libraries from a variety of cell types including primary euploid cell strains and somatic cell hybrids. Problems with chromosome recovery and maintenance of high molecular weight DNA have been resolved for applications involving YAC cloning. An exceptionally low rate of chimeras has been found in these flow sorted chromosome specific YAC libraries. Week-long continuous sorting experiments have been conducted to obtain sufficient material for cloning in large insert BAC libraries.
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MOLECULAR GENETICS OF MALE HYPERTENSION
MOLECULAR GENETICS OF MALE HYPERTENSION
APOPTOSIS VERSUS MALIGNANT PROGRESSION IN BREAST CANCER
CHROMOSOME SORTING FOR MULTISPECTRAL KARYOTYPING
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