APOPTOSIS VERSUS MALIGNANT PROGRESSION IN BREAST CANCER

乳腺癌细胞凋亡与恶性进展

• 批准号: 6470643
• 负责人: SCOTT CRAM
• 金额: $ 12.12万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6470643
• 关键词: biomedical resource; biotechnology; genetics; genome; human tissue; technology /technique

Georgetown University is studying the mechanisms of chromosomal instability in breast cancer, in particular the amplification or mutation of several oncogenes and suppresser genes. They are focused on the role of the myc oncogene in transgenic mouse models as a model system whereby they may study its role in mammary tumorigenesis. The specific aims are to obtain sorted mouse chromosomes from the FVB strain, fluorescently label these chromosomes to establish multicolor FISH spectral karyotyping at Georgetown, and to use this technique to establish the time dependent nature of chromosomal instability induced by the c-myc oncogene in mouse mammary epithelial cells.

乔治城大学正在研究染色体 乳腺癌的不稳定性，特别是扩增或 多个癌基因和抑制基因突变。 他们专注 关于myc癌基因在转基因小鼠模型中的作用 系统，从而他们可以研究其在乳腺肿瘤发生中的作用。 的 具体目的是从FVB获得分选的小鼠染色体 菌株，荧光标记这些染色体以建立染色体 FISH光谱核型分析，并使用这种技术， 确定诱导的染色体不稳定性的时间依赖性 c-myc癌基因在小鼠乳腺上皮细胞。