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MOUSE CHROMOSOME Y ANALYSIS & SORTING

MOUSE CHROMOSOME Y ANALYSIS & SORTING
小鼠染色体分析
批准号：
6119993
负责人：
SCOTT CRAM
金额：
$1.53万
依托单位：
UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
项目状态：
已结题
起止时间：
1999-07-01 至 2000-06-30
来源：
https://reporter.nih.gov/project-details/6119993
关键词：
biomedical resource biotechnology genome human tissue technology /technique

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英文摘要
IL-2 stimulated mouse spleen cells were used as a source of mouse chromosomes for analysis and sorting of the C57BL/6J mouse Y chromosome. The DNA content of this mouse Y chromosome is approximately 94.7 megabases, making it similar in size to human chromosome 16, and significantly larger than previous estimates. Two small insert Y chromosome-specific libraries, ideal for sampling Y chromosome sequences were constructed. Clone specificity was confirmed. From the characterized clones were developed 15 male-specific PCR assays, and 19 male-female PCR assays likely to originate from the pseudoautosomal region or other areas of X-Y or autosome-Y homology. (Genomics, 48, 304-313, 1998)
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MOLECULAR GENETICS OF MALE HYPERTENSION
MOLECULAR GENETICS OF MALE HYPERTENSION
APOPTOSIS VERSUS MALIGNANT PROGRESSION IN BREAST CANCER
CHROMOSOME SORTING FOR MULTISPECTRAL KARYOTYPING
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