HUMAN CHROMOSOME ANALYSIS & SORTING

人类基因组计划在一定程度上是由 对人类染色体进行分类的能力。 24个人中每一个最多25克 染色体类型正在被分类， 来自多种细胞类型的染色体特异性DNA文库 包括原代整倍体细胞株和体细胞杂种。 染色体恢复和高分子维持存在的问题 对于涉及YAC克隆的应用，已经解析了DNA的重量。 在这些流动中发现了异常低的嵌合体率 分选的染色体特异性YAC文库。 连续一周 已经进行了分选实验以获得足够的材料 用于在大插入BAC文库中克隆。

The human genome program has been facilitated, in part, by the ability to sort human chromosomes. Up to 2 5g of each of the 24 human chromosome types are being sorted for construction of chromosome-specific DNA libraries from a variety of cell types including primary euploid cell strains and somatic cell hybrids. Problems with chromosome recovery and maintenance of high molecular weight DNA have been resolved for applications involving YAC cloning. An exceptionally low rate of chimeras has been found in these flow sorted chromosome specific YAC libraries. Week-long continuous sorting experiments have been conducted to obtain sufficient material for cloning in large insert BAC libraries.