HUMAN CHROMOSOME ANALYSIS & SORTING

人类基因组计划得到了推动，部分原因是 能够对人类染色体进行排序。24个人中每个人最多25克 正在对染色体类型进行排序，以便构建 来自多种细胞类型的染色体特异DNA文库 包括原代整倍体细胞株和体细胞杂交种。 关于染色体恢复和维护的几个问题 重量DNA已经解决了涉及YAC克隆的应用。 在这些流动中发现了非常低的嵌合体比率 对染色体特异的YAC文库进行了分类。持续一周 已经进行了分选实验以获得足够的材料 用于在大插入BAC文库中克隆。

The human genome program has been facilitated, in part, by the ability to sort human chromosomes. Up to 2 5g of each of the 24 human chromosome types are being sorted for construction of chromosome-specific DNA libraries from a variety of cell types including primary euploid cell strains and somatic cell hybrids. Problems with chromosome recovery and maintenance of high molecular weight DNA have been resolved for applications involving YAC cloning. An exceptionally low rate of chimeras has been found in these flow sorted chromosome specific YAC libraries. Week-long continuous sorting experiments have been conducted to obtain sufficient material for cloning in large insert BAC libraries.