HUMAN CHROMOSOME ANALYSIS & SORTING

人类染色体分析

• 批准号: 6470658
• 负责人: SCOTT CRAM
• 金额: $ 12.12万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6470658
• 关键词: biomedical resource; biotechnology; genetics; genome; human tissue; technology /technique

The human genome program has been facilitated, in part, by the ability to sort human chromosomes. Up to 2 5g of each of the 24 human chromosome types are being sorted for construction of chromosome-specific DNA libraries from a variety of cell types including primary euploid cell strains and somatic cell hybrids. Problems with chromosome recovery and maintenance of high molecular weight DNA have been resolved for applications involving YAC cloning. An exceptionally low rate of chimeras has been found in these flow sorted chromosome specific YAC libraries. Week-long continuous sorting experiments have been conducted to obtain sufficient material for cloning in large insert BAC libraries.

人类基因组计划在一定程度上得到了促进 对人类染色体进行排序的能力。 24 人每人最多 2 5 克 染色体类型正在被分类以构建 来自多种细胞类型的染色体特异性 DNA 文库 包括原代整倍体细胞株和体细胞杂交体。 染色体恢复和高分子维持问题 重量 DNA 已解决涉及 YAC 克隆的应用。 在这些流程中发现嵌合体的比例极低 排序的染色体特异性 YAC 文库。 连续一周 进行了分选实验以获得足够的材料 用于在大型插入 BAC 文库中进行克隆。