MOUSE CHROMOSOME Y ANALYSIS & SORTING

用IL-2刺激的小鼠脾细胞作为小鼠的来源 C57BL/6J小鼠Y染色体的分析与分选 染色体。这条小鼠Y染色体的DNA含量是 大约94.7兆基数，使它的大小与人类相似 16号染色体，比之前估计的要大得多。二 小插入型Y染色体特异文库，适用于Y染色体取样 构建了染色体序列。克隆特异性是 确认了。从具有特征的克隆中开发出15个克隆 男性特异的PCR检测，以及19种男性-女性的可能 起源于假常染色体区域或X-Y或其他区域 常染色体-Y同源。(基因组学，48,304-313,1998)

IL-2 stimulated mouse spleen cells were used as a source of mouse chromosomes for analysis and sorting of the C57BL/6J mouse Y chromosome. The DNA content of this mouse Y chromosome is approximately 94.7 megabases, making it similar in size to human chromosome 16, and significantly larger than previous estimates. Two small insert Y chromosome-specific libraries, ideal for sampling Y chromosome sequences were constructed. Clone specificity was confirmed. From the characterized clones were developed 15 male-specific PCR assays, and 19 male-female PCR assays likely to originate from the pseudoautosomal region or other areas of X-Y or autosome-Y homology. (Genomics, 48, 304-313, 1998)