MOUSE CHROMOSOME Y ANALYSIS & SORTING

小鼠染色体分析

• 批准号: 6470655
• 负责人: SCOTT CRAM
• 金额: $ 12.12万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6470655
• 关键词: biomedical resource; biotechnology; genome; human tissue; technology /technique

IL-2 stimulated mouse spleen cells were used as a source of mouse chromosomes for analysis and sorting of the C57BL/6J mouse Y chromosome. The DNA content of this mouse Y chromosome is approximately 94.7 megabases, making it similar in size to human chromosome 16, and significantly larger than previous estimates. Two small insert Y chromosome-specific libraries, ideal for sampling Y chromosome sequences were constructed. Clone specificity was confirmed. From the characterized clones were developed 15 male-specific PCR assays, and 19 male-female PCR assays likely to originate from the pseudoautosomal region or other areas of X-Y or autosome-Y homology. (Genomics, 48, 304-313, 1998)

IL-2刺激的小鼠脾细胞被用作小鼠的来源 用于 C57BL/6J 小鼠 Y 分析和分选的染色体 染色体。 该小鼠 Y 染色体的 DNA 含量为 大约 94.7 兆碱基，与人类大小相似 16 号染色体，并且明显大于之前的估计。 二 小型插入 Y 染色体特异性文库，非常适合 Y 取样 构建了染色体序列。 克隆特异性为 确认的。 从表征的克隆中开发出 15 男性特异性 PCR 检测，以及 19 种男性-女性 PCR 检测可能 起源于伪常染色体区域或 X-Y 的其他区域或 常染色体-Y 同源性。 （基因组学，48, 304-313, 1998）