MOUSE CHROMOSOME Y ANALYSIS & SORTING

使用IL-2刺激的小鼠脾细胞作为小鼠脾细胞的来源。 C57 BL/6 J小鼠Y染色体的分析和分选 染色体 这只老鼠Y染色体的DNA含量是 大约94.7兆焦耳，使其大小与人类相似 第16号染色体，比以前的估计大得多。 两 小型插入Y染色体特异性文库，是Y染色体取样的理想选择 构建染色体序列。 克隆特异性为 确认 从所表征的克隆中开发了15个 男性特异性PCR检测，以及19种男性-女性PCR检测， 来源于假常染色体区域或X-Y的其他区域，或 常染色体Y同源性 （Genomics，48，304-313，1998）

IL-2 stimulated mouse spleen cells were used as a source of mouse chromosomes for analysis and sorting of the C57BL/6J mouse Y chromosome. The DNA content of this mouse Y chromosome is approximately 94.7 megabases, making it similar in size to human chromosome 16, and significantly larger than previous estimates. Two small insert Y chromosome-specific libraries, ideal for sampling Y chromosome sequences were constructed. Clone specificity was confirmed. From the characterized clones were developed 15 male-specific PCR assays, and 19 male-female PCR assays likely to originate from the pseudoautosomal region or other areas of X-Y or autosome-Y homology. (Genomics, 48, 304-313, 1998)