CORE--TRANSGENES AND GENE DISRUPTION IN MURINE MODELS

这个核心的目的是提供转基因动物， 采用转基因方法，用于项目1-5。老鼠会 通过注射DNA受精卵或注射获得 胚胎干细胞(ES细胞)进入囊胚。胚胎干细胞携带 通过同源重组获得靶向突变。其核心是 接收携带靶向突变的可注射DNA或ES细胞 并为他们提供转基因或嵌合体 供进一步饲养的小鼠。此外，核心将协调 并提供细胞系、指导、建议和培训 调查人员将确保高效地产生转基因菌株 成功率很高。

The purpose of this core is to provide genetically modified animals, employing transgenic methodologies, for use in Projects 1-5. Mice will be obtained either via DNA injection of fertilized eggs or via injection of embryonic stem cells (ES cells) into blastocysts. The ES-cells carry targeted mutations obtained via homologous recombination. The core receives injection-ready DNA or ES-cells carrying targeted mutations from the different investigators and provides them with transgenic or chimeric mice for further breeding. In addition, the core will coordinate the injections and provide cell lines, guidance, advice and training to investigators to ensure efficient generation of the transgenic strains with a high success rate.