CORE--TRANSGENES AND GENE DISRUPTION IN MURINE MODELS

这个核心的目的是提供转基因动物， 采用转基因方法，用于项目1-5。 小鼠将 通过受精卵的DNA注射或通过注射 胚胎干细胞（ES细胞）进入囊胚。 ES细胞携带 通过同源重组获得的靶向突变。 核心 接受注射准备好的DNA或携带靶向突变的ES细胞 并为他们提供转基因或嵌合的 小鼠进一步繁殖。 此外，核心将协调 注射和提供细胞系，指导，建议和培训， 研究人员确保转基因菌株的有效产生 成功率很高。

The purpose of this core is to provide genetically modified animals, employing transgenic methodologies, for use in Projects 1-5. Mice will be obtained either via DNA injection of fertilized eggs or via injection of embryonic stem cells (ES cells) into blastocysts. The ES-cells carry targeted mutations obtained via homologous recombination. The core receives injection-ready DNA or ES-cells carrying targeted mutations from the different investigators and provides them with transgenic or chimeric mice for further breeding. In addition, the core will coordinate the injections and provide cell lines, guidance, advice and training to investigators to ensure efficient generation of the transgenic strains with a high success rate.