CORE--TRANSGENIC AND GENE DISRUPTION LABORATORY

核心——转基因和基因破坏实验室

• 批准号: 6595010
• 负责人: GERARD C GROSVELD
• 金额: $ 24.14万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6595010
• 关键词: animal breeding; animal colony; biomedical facility; gene mutation; genetically modified animals; laboratory mouse

The purpose of this core is to provide genetically modified animals, employing transgenic methodologies, for use in Projects 1-4. Mice will be obtained either via DNA infection of fertilized eggs or via injection of embryonic stem cells (ES cells) into blastocysts. The ES-cells carry targeted mutations obtained via homologous recombination. The core receives injection-ready DNA or ES-cells carrying targeted mutations from the different investigators and provide them with transgenic or chimeric mice for further breeding. In addition, the core will coordinate the injections and provide cell lines, guidance, and advice to investigators to ensure efficient generation of the transgenic strains.

该核心的目的是提供采用转基因方法的转基因动物，用于项目1-4。小鼠将通过受精卵的DNA感染或通过将胚胎干细胞（ES细胞）注射到囊胚中获得。ES细胞携带通过同源重组获得的靶向突变。核心接收来自不同研究者的携带靶向突变的注射就绪DNA或ES细胞，并为他们提供转基因或嵌合小鼠以供进一步繁殖。此外，核心将协调注射，并为研究人员提供细胞系，指导和建议，以确保转基因菌株的有效产生。