Transgenic Gene Knockout

转基因基因敲除

• 批准号: 8738010
• 负责人: GERARD C GROSVELD
• 金额: $ 0.08万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8738010
• 关键词: Animal Model; Cancer Center; Cancer Center Support Grant; Cell Differentiation process; DNA; Genes; Injection of therapeutic agent; Malignant Neoplasms; Mouse Strains; Mus; Mutate; Principal Investigator; Research Personnel; Resource Sharing; Retroviridae; Technology; Time; Transgenic Organisms; blastocyst; cell growth; embryonic stem cell; gene function; in vivo; knockout gene; zygote

Animal models created by transgenic and gene knockout technology are invaluable in evaluating gene function in vivo and in determining how certain genes regulate cell growth and differentiation and how alterations in these genes contribute to cancer. Mice are genetically modified in this facility either by pronucleus injection of DNA into fertilized eggs, by injection of retroviruses into the perivitteline space of fertilized eggs, or by injection of mutated embryonic stem cells (ES-cells) into blastocysts. The objective of this shared resource is to provide genetically modified mice to principal Investigators within the Cancer Center in the most time- and costeffective way. In addition, the availability of these mouse strains greatly stimulates interactions among investigators at St Jude.

转基因和基因敲除技术创建的动物模型对于评估基因功能具有不可估量的价值 体内和确定某些基因如何调节细胞生长和分化以及如何改变 这些基因会导致癌症。在该设施中通过原核注射对小鼠进行基因改造 通过将逆转录病毒注射到受精卵的卵周间隙，或通过注射将 DNA 注入受精卵 将突变的胚胎干细胞（ES 细胞）转化为囊胚。该共享资源的目标是提供 以最省时、最具成本效益的方式向癌症中心的主要研究人员提供转基因小鼠 方式。此外，这些小鼠品系的可用性极大地刺激了小鼠之间的相互作用 圣裘德的调查员。