Transgenic Gene Knockout

转基因基因敲除

• 批准号: 7714166
• 负责人: GERARD C GROSVELD
• 金额: $ 16.37万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-14 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714166
• 关键词: Animal Model; Cancer Center; DNA; Genes; Injection of therapeutic agent; Malignant Neoplasms; Mouse Strains; Mus; Mutate; Principal Investigator; Research Personnel; Resource Sharing; Retroviridae; Technology; Time; Transgenic Organisms; blastocyst; cell growth; embryonic stem cell; gene function; in vivo; knockout gene; zygote

Animal models created by transgenic and gene knockout technology are invaluable in evaluating gene function in vivo and in determining how certain genes regulate cell growth and differentiation and how alterations in these genes contribute to cancer. Mice are genetically modified in this facility either by pronucleus injection of DNA into fertilized eggs, by injection of retroviruses into the perivitteline space of fertilized eggs, or by injection of mutated embryonic stem cells (ES-cells) into blastocysts. The objective of this shared resource is to provide genetically modified mice to principal Investigators within the Cancer Center in the most time- and costeffective way. In addition, the availability of these mouse strains greatly stimulates interactions among investigators at St Jude.

利用转基因和基因敲除技术建立的动物模型在评估基因功能方面具有不可估量的价值