Core--Instrumental and analytical measurements

核心——仪器和分析测量

• 批准号: 6315346
• 负责人: Ulrich Hopfer
• 金额: $ 25.95万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6315346
• 关键词: biomedical facility; confocal scanning microscopy; electroporation; fluorescence microscopy; intracellular transport; microscopy; protein localization

The Instrumentation Core will provide all projects and principle investigators of this Program Project Grant with: a) confocal and conventional light microscopy and image analyses of fixed samples for localization and colocalization experiments, as well as quantification of morphological changes. b) ratiometric fluorescence microscopy of live cells for dynamic measurements of intracellular ion concentration of membrane potential changes. c) microinjection of proteins and nucleic acids to modulate intracellular message or signaling systems. d) injection of proteins into cells by electroporation to effect modulation of intracellular signaling system in biochemical studies. e) routine resistance and potential measurements of epithelial monolayers as quality control. f) quantitative analyses of protein and nucleic aid gels and immuno-blots. The staff of the Core will carry out measurements and provide assistance and counseling in planning of measurements and data analyses.

仪器核心将为所有项目和本计划项目资助的主要研究人员提供：a）共聚焦和常规光学显微镜和固定样品的图像分析，用于定位和共定位实验，以及形态变化的量化。B）活细胞的比率荧光显微术，用于膜电位变化的细胞内离子浓度的动态测量。c）显微注射蛋白质和核酸以调节细胞内信息或信号系统。d）通过电穿孔将蛋白质注射到细胞中以在生物化学研究中实现细胞内信号传导系统的调节。e）作为质量控制的上皮单层的常规电阻和电位测量。f）蛋白质和核酸凝胶和免疫印迹的定量分析。该中心的工作人员将进行测量，并在测量和数据分析的规划方面提供援助和咨询。