Core--Instrumental and analytical measurements

核心——仪器和分析测量

• 批准号: 6589303
• 负责人: Ulrich Hopfer
• 金额: $ 25.95万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6589303
• 关键词: biomedical facility; confocal scanning microscopy; electroporation; fluorescence microscopy; intracellular transport; microscopy; protein localization

The Instrumentation Core will provide all projects and principle investigators of this Program Project Grant with: a) confocal and conventional light microscopy and image analyses of fixed samples for localization and colocalization experiments, as well as quantification of morphological changes. b) ratiometric fluorescence microscopy of live cells for dynamic measurements of intracellular ion concentration of membrane potential changes. c) microinjection of proteins and nucleic acids to modulate intracellular message or signaling systems. d) injection of proteins into cells by electroporation to effect modulation of intracellular signaling system in biochemical studies. e) routine resistance and potential measurements of epithelial monolayers as quality control. f) quantitative analyses of protein and nucleic aid gels and immuno-blots. The staff of the Core will carry out measurements and provide assistance and counseling in planning of measurements and data analyses.

仪器核心将为本项目的所有项目和主要研究人员提供：a)用于定位和共定位实验的共聚焦和常规光学显微镜以及固定样品的图像分析，以及形态变化的量化。B)用比例荧光显微镜动态测量活细胞内离子浓度的膜电位变化。C)微量注射蛋白质和核酸来调节细胞内信息或信号系统。D)在生化研究中，通过电穿孔将蛋白质注射到细胞中来调节细胞内信号系统。E)常规耐药性和上皮单层电位测量作为质量控制。F)蛋白和核酸凝胶和免疫印迹的定量分析。核心工作人员将进行测量，并在规划测量和数据分析方面提供协助和咨询。