LIGHT MICROSCOPY IMAGING SYSTEM

凯斯西储大学的囊性纤维化中心拥有专门的多用户成像设备，用于对活细胞进行三维和四维细胞生理研究。这些研究涉及荧光探针检测到的细胞过程的动态测量。研究的过程包括细胞内信号事件、跨上皮电解质转运、细胞炎症反应、受体介导的基因转移和粘蛋白胞吐。这些研究需要比现有系统更好的时间和空间分辨率。因此，我们需要一种光学显微镜成像系统，该系统能够以更高的空间和时间分辨率对活细胞进行荧光研究，以及对仪器和数据采集的计算机控制。选择的系统是基于蔡司Axiovert 100米的成像站，具有基于尼普考圆盘的共焦能力。该系统将为获取具有足够时间分辨率的3D图像提供硬件和软件，并通过从一叠明亮的荧光图像以及从共焦图像(尼普考光盘系统)进行图像恢复来进行离线重建。由于更高的时间和空间分辨率，新的显微镜系统将为正在进行的资助研究提供重大增强和加速，在这些研究中，关于活细胞过程的动态和细胞定位的信息是重要的。

The Cystic Fibrosis Center at Case Western Reserve University maintains a specialized multiuser imaging facility for three dimensional and 4 dimensional cell physiological studies on live cells. These studies are concerned with dynamic measurements of cellular processes as detected by fluorescent probes. Processes studied include intracellular signaling events, transepithelial electrolyte transport, cellular inflammatory responses, receptor-mediated gene transfer, and mucin exocytosis. These studies require better temporal and spatial resolution than is possible with the current system. Therefore, we request a Light Microscopy Imaging system that allows fluorescence studies on live cells with greater spatial and temporal resolution, as well as computer control of the instrumentation and data acquisition. The system of choice is a Zeiss Axiovert 100M based imaging station with Nipkow-disc based confocal capability. The system will provide the hard- and software for 3D image acquisition with sufficient temporal resolution and off-line reconstructions by image restoration from a stack of brightfield epifluorescence pictures as well as from confocal images (Nipkow disc system). Due to the greater temporal and spatial resolution, the new microscopy system will provide major enhancements and acceleration of ongoing funded research in which information on the dynamic and cellular localization of processes in live cells is important.