CORE--CELL PHYSIOLOGY

细胞生理学核心通过提供访问 视频增强显微镜和常规经上皮 电生理学 核心负责维护这些设施和列车 调查人员在使用。 视频显微镜设置包括 具有落射荧光、DIC和相位光学的正置显微镜。 的 可以通过传统的高分辨率同时捕获图像 摄像机（透射图像）和增强CCD摄像机（用于 荧光），连续数字化和在线分析， 荧光软件包。 滤光轮可以改变波长 激发荧光，使细胞内钙或pH 通过荧光探针和比率法进行测量。 光子模式 特别适用于低荧光分析 免疫细胞化学 该核心将广泛用于细胞溶质 项目#1、#3和#4的Cl、Na、K和pH值测量，以及 通过项目#2、#4和5进行免疫荧光。 项目#3和#5将 还利用测量短路电流的能力， 在多孔过滤器上生长的汇合单分子膜的电势和电导 支持.

The Cell Physiology Core supports investigators by providing access to video-enhanced microscopy and conventional transepithelial electrophysiology. The Core maintains these facilities and trains investigators in their use. The video microscopy set-up includes an upright microscope with epifluorescence and DIC and phase optics. The images can be captured simultaneously by a conventional, high resolution video camera (transmission image) and an intensified CCD camera (for fluorescence), continuously digitized and analyzed on-line by fluorescence software package. A filter wheel can change the wavelength of excitation for fluorescence, enabling intracellular Ca or pH measurements by fluorescent probes and the ratio method. A photonic mode is particularly well suited for the analysis by low-fluorescence immunocytochemistry. This core will be extensively used for cytosolic Cl, Na, K, and pH measurements by Projects #1, #3, and #4, and also for immunofluorescence by Projects #2, #4, and 5. Projects #3 and 5 will also take advantage of the capability to measure short-circuit current, potential, and conductance of confluent monolayers grown on porous filter supports.