CORE--CELL PHYSIOLOGY

细胞生理学核心通过提供 获得视频增强的光学显微镜和上皮细胞 电生理学，不仅是对直接参与CF的研究人员 研究，但也适用于其他从事肺部研究或上皮细胞研究的人 电解质运输，因为这些区域是相关的，也是相关的 对于慢性萎缩性胃炎患者。成像设备由两个完整的显微镜组成 设置图像采集、分析软件、数据存储和 维持活细胞的适当设备(受控环境 和温度)外加一个额外的分析站：1)直立式蔡司 配备荧光的ACM显微镜，一种增强型电荷耦合器件 相机和环球成像公司的1/FL PC软件。2)倒置的蔡司 带有自动滤光轮、工作台和焦点的Axiovert 35显微镜，a 生物制冷CCD摄像机和基于Macintosh的成像软件 探测系统。电生理学设施由一个 保温箱和电压钳，也配有受控环境 和温度，以及Millicel ERS探测器。视频增强的灯光 显微镜主要用于动态调节实验。 细胞内离子浓度的荧光指示剂(钙， 氯、钠、pH)，用于荧光标签的共同定位，例如不同地 标记抗体，并用于三维可视化(3-D 重建)。电压钳设置(Ussing小室)用于 人上皮细胞单层的详细电生理分析 或整个上皮组织的过滤器(在改进的Ussing小室中)，以及 Millicel ERS探头用于常规测量 跨上皮电导。核心维护和改进 仪器，为用户提供培训和监督使用 这些技术还提供质量控制，并成为 参与调查人员之间的思想交流。核心收费 使用成像设施的费用，以支付部分 维修和更换旧的或过时的设备的费用 成像设施，并鼓励对此进行深思熟虑的利用 资源。Core的可用性不仅为调查人员提供了 可以使用技术专业化且昂贵的仪器，但允许 他们把精力集中在感兴趣的生物学问题上 而不是仪器的技术方面。这个 CF研究中心研究人员对该核心的利用情况 在过去五年中，员工数量大幅增加，因为 出版物数量和赠款支助的增加证明了这一点。

The Cell Physiology Core supports the CF Research Center by providing access to video-enhanced light microscopy and epithelial electrophysiology, not only to researchers directly involved with CF research, but also, to others working on pulmonary research or epithelial electrolyte transport, as these areas are related and also have relevance for CF patients. The imaging facility consists of two complete microscope set-ups with image acquisition, analysis software, data storage, and the appropriate equipment for maintaining live cells (controlled environment and temperature) plus one additional analysis station: 1) an upright Zeiss ACM microscope that is equipped with epifluorescence, an intensified CCD camera and Universal Imaging's 1/FL PC-software. 2) An inverted Zeiss Axiovert 35 microscope with automated filter wheels, stage, and focus, a cooled CCD camera and Macintosh based imaging software from Biological Detection Systems. The electrophysiology facility consists of an incubator and a voltage clamp, also equipped with controlled environment and temperature, and Millicel ERS probes. The video-enhanced light microscopes are used predominantly for dynamic experiments of regulation of intracellular ion concentrations with fluorescent indicator dyes (Ca, Cl, Na, pH), for co-localization of fluorescent tags, e.g., differently labeled antibodies, and for 3-dimensional visualization (3-D reconstruction). The voltage clamp set-up (Ussing chamber) is used for detailed electrophysiological analysis of epithelial cell monolayers on filters or of whole epithelial tissues (in a modified Ussing chamber), and the Millicel ERS probes are used for routine measurements of transepithelial conductance. The Core maintains and improves the instrumentation, provides training and supervision of users in the use of the technologies, also provides quality control and serves as a focus for the exchange of ideas among participating investigators. The Core charges a fee for the use of the imaging facility in order to defray part of the cost of repairing and replacing either worn or outdated equipment of the imaging facility, and to encourage thoughtful utilization of this resource. The availability of the Core not only gives investigators access to technically specialized and expensive instrumentation but allows them to concentrate their efforts on the biological questions of interest rather than on the technical aspects of the instrumentation. The utilization of this Core by the CF Research Center investigators and associates has increased substantially over the past five years as evidenced by the increased number of publications and grant support.