BLOOD OXYGEN LEVEL DEPENDENT (BOLD) MR IMAGING & BRAIN OXYGEN TENSION

血氧水平相关（粗体）MR 成像

• 批准号: 6353202
• 负责人: Jeffrey Frank Dunn
• 金额: $ 1.22万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353202
• 关键词: Mammalia; biomedical resource; cardiovascular system; drug /agent; gas; injury; urinary tract

Renal ischemia is a major cause of acute renal failure. Ulinastatine (UTI), a protease inhibitor, has been reported to help protect against ischemia and various types of shock; however, its mechanism for these protective effects is not fully known. We have investigated the effects of UTI on renal ischemia and reperfusion by measuring the tissue pO2 simultaneously in the renal cortex and medulla in rats. The principal experimental protocol was 30 min renal ischemia (by clamping the abdominal aorta) followed by 60 min reperfusion. Treatments were 0.9%NaCl 1ml/kg (NS group) or UTI 50000U/1ml/kg (UTI group) administered 30 min before ischemia. In the NS group, the control pO2 values of renal cortex and medulla were 11.7 +/- 2.3 and 14.4 +/- 5.7 mmHg respectively, whereas those in the UTI group were 18.9 +/- 4.0 and 24.5 +/- 4.2 mmHg. The pO2 of the cortex and medulla decreased to less than 2 mmHg during ischemia in both groups. At 60 min after reperfusion, the pO2 values in NS group were not fully restored (7.6 +/- 2.3 and 6.9 +/-1.4 mmHg), whereas those in the UTI group were completely restored to the control value (21.1 +/-4.8 and 25.5 +/- 2.1 mmHg) . These data indicate that administration of UTI before ischemia increases the renal cortical and medullary pO2 after reperfusion.

肾脏缺血是急性肾衰竭的主要原因。 据报道，Ulinastatine（UTI）是一种蛋白酶抑制剂可帮助 预防缺血和各种冲击；但是，它 这些保护作用的机制尚不清楚。 我们有 研究了UTI对肾脏缺血的影响和通过 同时在肾皮质中测量组织PO2和 老鼠的髓质。 主要实验方案为30分钟肾脏 缺血（通过夹紧腹主动脉），然后进行60分钟 再灌注。 治疗为0.9％NaCl 1ml/kg（NS组）或UTI 50000U/1ml/kg（UTI组）在缺血前30分钟施用。 在 NS组，肾皮质和髓质的控制PO2值为11.7 +/- 2.3和14.4 +/- 5.7 mmHg，而UTI中的+/- 组为18.9 +/- 4.0和24.5 +/- 4.2 mmHg。 皮质的PO2 在缺血期间，延髓降低到小于2 mmHg 组。 再灌注后60分钟，NS组的PO2值为 未完全恢复（7.6 +/- 2.3和6.9 +/- 1.4 mmHg），而这些 UTI组完全恢复为控制值（21.1 +/- 4.8和25.5 +/- 2.1 mmHg）。 这些数据表明 局部缺血之前的UTI增加了肾皮质和 再灌注后的髓质PO2。