MOUSE T CELL RECEPTORS SPECIFIC FOR VESICULAR STOMATITIS VIRUS & HIV CD4 STRUCT

水泡性口炎病毒特异性小鼠 T 细胞受体

• 批准号: 6310155
• 负责人: ELLIS L REINHERZ
• 金额: $ 5.83万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6310155
• 关键词: AIDS; animal tissue; bioimaging /biomedical imaging; biological products; biomedical resource; immunology; infection; inflammation; lymphatic system; radiography; radiology; structural biology; virus

To solve the three dimensional structures of multiple murine T cell receptors specific for a viral octapeptide (VSV8) bound to the K b MHC class I molecule T cells recognize cell-associated pathogens of viral, bacteria[ or fungal origin as well as tumor antigens through a unique molecule termed the T cell receptor (TCR). The TCR complex consists of multiple transmembrane chains on the surface of T lymphocytes',". The disulfide-linked a-P heterodimer is a clonally unique component that possesses a recognition site for antigen in the context of the major histocompatibility complex (MHC), while the invariant CD3 components (y, 6, e, and Q are involved in signal transduction. Because of the intimate membrane association of this complex, study of the molecular features of TCR recognition necessarily have been indirect. Recent efforts to understand the nature of this recognition process have focused on structural studies of the system. It has been known for a few years that both class I and class 11 MHC molecules, despite the very different chemical compositions of the two subunits, have the same topologic structure"-s. in particular, the way MHC molecules present the antigenic peptide is similar. A short antigenic peptide (8-10 residues for class I and >1 2 residues for class 11) lies in a large groove at the top of the MHC molecule. This groove is composed of eight strands of anti-parallel P-sheet as a floor, and two anti-parallel a-helices as side walls. In class I MHC molecules, the whole groove is formed by domain al and a2 from the same a chain; in class 11 MHC molecules, the groove is formed by domain al, and 01 from two different chains (a and 0). Structural details of how peptides bind with high affinity to these MHC molecules have been well characterized.

为了解决多个小鼠T细胞的三维结构， 特异性针对结合至K的病毒八肽（VSV 8）的细胞受体 B类MHC I类分子 T细胞识别与细胞相关的病毒、细菌[或 真菌来源以及肿瘤抗原通过一个独特的分子 称为T细胞受体（TCR）。 TCR复合物由以下组成： T淋巴细胞表面的多个跨膜链”。 的 二硫键连接的α-P异源二聚体是克隆独特的组分， 具有抗原的识别位点， 组织相容性复合物（MHC），而不变的CD 3成分 （y、6、e和Q参与信号转导。 因为 这种复合物的紧密膜缔合， TCR识别的特征必然是间接的。 最近为了解这一承认过程的性质所作的努力 重点是系统的结构研究。 人们已经知道 几年来，I类和11类MHC分子，尽管 这两种亚基的化学组成非常不同， 相同的拓扑结构”-s。 尤其是MHC分子 目前的抗原肽是相似的。 一种短的抗原肽 (8-10 I类的> 12个残基和11类的> 12个残基）位于 MHC分子顶端的大凹槽。 这个凹槽是由 八股反平行的P片作为地板， 反平行α-螺旋作为侧壁。 在I类MHC分子中， 整个凹槽由来自相同α链的结构域α 1和α 2形成; 在第11类MHC分子中，沟由结构域a1和01形成， 两条不同的链（a和0）。 肽如何在细胞中 与这些MHC分子的高亲和力结合已经很好地 表征了