MULTIPHOTON MICROSCOPE FOR PURDUE UNIVERSITY

普渡大学多光子显微镜

• 批准号: 6291360
• 负责人: JOSEPH PAUL ROBINSON
• 金额: $ 50万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2003-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291360
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; microscopy

This application for a multiphoton microscope builds upon the past six years of developing confocal microscopy applications in the Cancer Center and across the Purdue University campus. There are currently no multiphoton microscopes at this university. Over the past few years, a steady number of requests to perform experiments that exceeded the current confocal capability, required us to consider a more advanced technology. Two specific areas of experimentation created significant difficulties for our current confocal microscope system: deep tissue imaging and analysis of live cell systems in a kinetic protocol. The deep tissue imaging became a recurrent theme of the requests to use the confocal and culminated in the conclusion that we had reached the abilities of our current instrument. Our current instrument is still a very important instrument. Indeed, it has been a tremendous resource and we anticipate a continuation of the use of this instrument. Specifically it has become the instrument of choice for reflection imaging and will continue to play a significant role in the campus's biological imaging program. The second area of difficulty faced by our confocal users was the long term imaging of live cells. These assays include functional assays, observations of dividing or differentiation cells, phenotyping deep tissue cells, and functional analysis of cells including calcium flux. Live cell studies are in strong demand and will be difficult to perform adequately with our present system. The instrument requested will bring to Purdue University a capability it does not presently possess. We have waited for nearly 3 years, since originally considering our need for multiphoton. While we had demonstrated uses three years ago, it was our opinion that the technology was then too immature. Present-day almost turn-key systems are more desirable in our environment. Funding of this application will bring a significant advance to our present confocal capabilities.

多光子显微镜的这一应用建立在过去六年在癌症中心和普渡大学校园开发共焦显微镜应用的基础上。该大学目前没有多光子显微镜。在过去的几年里，不断有越来越多的要求进行超出当前共焦能力的实验，这要求我们考虑更先进的技术。两个特定的实验领域给我们当前的共焦显微镜系统带来了重大困难：深层组织成像和动态协议中活细胞系统的分析。深层组织成像成为使用共聚焦的请求中反复出现的主题，最终得出的结论是我们已经达到了当前仪器的能力。我们现在的仪器仍然是一个非常重要的仪器。事实上，它是一个巨大的资源，我们期望继续使用该工具。具体来说，它已成为反射成像的首选仪器，并将继续在校园的生物成像项目中发挥重要作用。我们的共聚焦用户面临的第二个困难是活细胞的长期成像。这些测定包括功能测定、分裂或分化细胞的观察、深层组织细胞的表型分析以及细胞的功能分析（包括钙通量）。 Live cell studies are in strong demand and will be difficult to perform adequately with our present system.所要求的工具将为普渡大学带来目前不具备的能力。从最初考虑多光子的需求开始，我们已经等了近三年了。虽然我们在三年前就已经展示了用途，但我们认为当时的技术还太不成熟。目前几乎交钥匙的系统在我们的环境中更受欢迎。该应用程序的资助将为我们目前的共聚焦能力带来重大进步。