A REPLACEMENT CELL SORTER FOR PURDUE UNIVERSITY

此应用程序将购买新的细胞分选器，以取代现有的旧分选器，该分选器不再可靠，无法升级到当前标准，并且不能满足当前用户的许多要求。在过去的14年里，普渡大学一直在维持细胞分选操作。1986年，美国国立卫生研究院共享仪器拨款购买了一架EPICS 752。这台仪器于1994年完全停止使用。1990年，根据与制造商的一项协议，我们获得了EPICS Elite分拣机的原型，在过去的9年里，它在这个设施中发挥了很好的作用。因为这个仪器不是一个完整的生产模型，所以仪器的某些方面不是标准的。此外，该仪器的使用寿命即将结束，随着我们机构对流式细胞仪分选方面的额外要求，我们认为是时候用一种能够满足我们用户需求的仪器来取代该仪器了。在这方面，建议的仪器不仅设计为执行高速分拣，而且进行快速设置分拣，一个范围从12-384井板的优秀单细胞板沉积单元，以及我们目前需要或可能在未来几年内与当前和未来用户需要的所有激光组合。该设施在共享使用我们的高科技仪器方面有着长期的记录。他们所在的资源具有丰富的历史，不仅为该机构的NIH调查人员提供资源，而且为全国各地机构的调查人员提供知识和教育资源。所要求的仪器将为我们的资源提供急需的升级，为NIH资助的研究人员提供生产高质量科学所需的高质量工具。

This application is to purchase a new cell sorter to replace an existing, older sorter which is no longer reliable, cannot be upgraded to current standards, and cannot perform a number of requirements of current users. Over the past 14 years Purdue University has maintained a cell sorter operation. In 1986 an Epics 752 was purchased under an NIH shared instrumentation grant. This instrument was taken completely out of service in 1994. In 1990, under an agreement with the manufacturer, we obtained a prototype Epics Elite sorter which has served this facility well over the past 9 years. Because this instrument was not a full production model, there are certain aspects to the instrument that are not standard. In addition, the instrument is close to reaching the end of its useful life and, with added demands upon the sorting aspects of flow cytometry at our institution, we believe it is time to replace this instrument with an instrument that can accommodate our user's needs. In this regard the instrument proposed has been designed to perform not only high speed sorts, but rapid set-up sort sessions, an excellent single cell plate deposition unit ranging from 12-384 well plates, and all of the laser combinations we currently require or &e likely to need with current and future users over the next several years. The facility has a long track record of shared use of our high technology instruments. The resource in which they are operated has a rich history of not only providing resources to NIH investigators at this institution, but also knowledge and educational resources to those at institutions all over the country. The instrument requested will provide a much needed upgrade to our resource providing NIH funded investigators with the high quality tools necessary to produce quality science.