Laser Scanning Cytometer for Purdue University

描述(由申请者提供)：在过去的15年里，普渡大学的流式细胞仪能力已经成熟。自1987年以来，我们升级了我们的流式细胞仪和成像仪器，包括SIG(1993、2000、2001)的支持，使我们能够保持对大学教职员工的高质量服务和仪器。自1988年以来，普渡大学细胞学实验室(PUCL)一直为教职员工提供持续的服务。在过去的15年里，超过366名教职员工使用了该设施。已有300多名研究生接受了高级培训。然而，PUCL设施不仅仅是为研究人员提供仪器支持。一旦仪器就位，我们将提供出色的技术支持和质量建议。我们拥有4名全职技术人员和1名软件专家，他们的整个角色是为调查人员提供高质量的支持。进入该设施的仪器设备不仅保证对教职员工可用，而且按承诺提供高质量的服务。我们成功地向许多不知道其功能的教职员工介绍了流式细胞术，并通过参与我们的SIG赠款来支持那些推动我们计划的人。我们现在发现我们自己受到了细胞学的重大发展的挑战-功能分析更需要在细胞培养和组织中的细胞上进行参数化的形态和亚细胞定位以及基因表达，同时保持空间相关性。Compucyte iCyte成像细胞仪是一种商业上可用的细胞仪，它提供了这项申请申请资金所需的功能。尽管由4名初级用户和6名二级用户推动，但我们的历史记录预测，随着我们在运营和管理方面的经验成熟，它将被更多的教职员工使用。将共享资源放置到一个在共享和支持方面有着良好记录的设施中，是支持该机构NIH资助的研究人员的一种经济高效的方式。

DESCRIPTION (provided by applicant): Flow cytometry capabilities have been maturing at Purdue University over the past 15 years. Since 1987, we have upgraded our flow cytometers and imaging instruments including support from SIG (1993, 2000, 2001) which allowed us to maintain high quality services and instruments to the faculty of the university. Since 1988, the Purdue University Cytometry Laboratories (PUCL) has maintained continuous service to the faculty. Over 366 faculty have used the facility in the past 15 years. Over 300 graduate students have been trained at advanced levels. The PUCL facility however, is not just about providing instrument support to researchers. Once the instruments are in place, we provide excellent technical support and quality advice. We maintain 4 full time technicians and a software expert whose entire role is to provide quality support to investigators. Instrumentation that comes into this facility is guaranteed to be not only available to faculty, but is provided a la carte with a commitment to quality service. We successfully introduced flow cytometry to many faculty who were not aware of its capability and supported those who have driven our program by participating in our SIG grants. We now find ourselves challenged by the significant development of cytomics - functional analysis with a higher need for parameterized morphology and sub-cellular localization and gene expression on cells in cell cultures and in tissue while maintaining spatial correlations. The Compucyte iCyte Imaging Cytometer is a commercially available cytometer that offers this capability for which this application requests funding. Although driven by 4 primary and 6 secondary users, our track record predicts that it will be used by many more faculty as our experience in its operation and management matures. Placement of a shared resource into a facility that has a strong track record of sharing and support is a cost effective way of supporting NIH funded researchers at this institution.