Laser Scanning Cytometer for Purdue University

普渡大学激光扫描细胞仪

• 批准号: 6876911
• 负责人: JOSEPH PAUL ROBINSON
• 金额: $ 34.2万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876911
• 关键词: biomedical equipment purchase; biomedical facility; biomedical resource; flow cytometry; health science research support; molecular /cellular imaging

DESCRIPTION (provided by applicant): Flow cytometry capabilities have been maturing at Purdue University over the past 15 years. Since 1987, we have upgraded our flow cytometers and imaging instruments including support from SIG (1993, 2000, 2001) which allowed us to maintain high quality services and instruments to the faculty of the university. Since 1988, the Purdue University Cytometry Laboratories (PUCL) has maintained continuous service to the faculty. Over 366 faculty have used the facility in the past 15 years. Over 300 graduate students have been trained at advanced levels. The PUCL facility however, is not just about providing instrument support to researchers. Once the instruments are in place, we provide excellent technical support and quality advice. We maintain 4 full time technicians and a software expert whose entire role is to provide quality support to investigators. Instrumentation that comes into this facility is guaranteed to be not only available to faculty, but is provided a la carte with a commitment to quality service. We successfully introduced flow cytometry to many faculty who were not aware of its capability and supported those who have driven our program by participating in our SIG grants. We now find ourselves challenged by the significant development of cytomics - functional analysis with a higher need for parameterized morphology and sub-cellular localization and gene expression on cells in cell cultures and in tissue while maintaining spatial correlations. The Compucyte iCyte Imaging Cytometer is a commercially available cytometer that offers this capability for which this application requests funding. Although driven by 4 primary and 6 secondary users, our track record predicts that it will be used by many more faculty as our experience in its operation and management matures. Placement of a shared resource into a facility that has a strong track record of sharing and support is a cost effective way of supporting NIH funded researchers at this institution.

描述（由申请人提供）：在过去的15年中，普渡大学的流式细胞术能力已经成熟。自1987年以来，我们已经升级了我们的流式细胞仪和成像仪器，包括SIG（1993年，2000年，2001年）的支持，使我们能够保持高质量的服务和仪器大学的教师。自1988年以来，普渡大学细胞测量实验室（PUCL）一直保持持续服务的教师。在过去的15年里，超过366名教师使用了该设施。300多名研究生接受了高级培训。然而，PUCL设施不仅仅是为研究人员提供仪器支持。一旦仪器到位，我们将提供出色的技术支持和高质量的建议。我们有4名全职技术人员和一名软件专家，他们的全部职责是为调查人员提供高质量的支持。进入这个设施的仪器保证不仅提供给教师，但提供了一个点菜的承诺，以优质的服务。我们成功地将流式细胞术介绍给了许多不了解其能力的教师，并支持那些通过参与我们的SIG赠款来推动我们计划的人。我们现在发现自己受到细胞组学的重大发展的挑战-功能分析，更需要在细胞培养物和组织中的细胞上参数化形态和亚细胞定位以及基因表达，同时保持空间相关性。Compucyte iCyte成像细胞仪是一种市售细胞仪，可提供本申请所需资金的这种功能。虽然由4个主要用户和6个次要用户驱动，但我们的记录预测，随着我们在运营和管理方面的经验成熟，它将被更多的教师使用。将共享资源放置到一个具有强大的共享和支持记录的设施中，是支持该机构NIH资助研究人员的一种具有成本效益的方式。