Core--Analytical cytology

核心--分析细胞学

• 批准号: 6660906
• 负责人: JOSEPH PAUL ROBINSON
• 金额: $ 21.7万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660906
• 关键词: DVD /CD ROM; bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; confocal scanning microscopy; flow cytometry; neoplasm /cancer; oncology

The Analytical Cytology Shared Resource provides flow cytometry, image analysis and confocal microscopy facilities to Cancer Center members. The flow cytometry facilities include an Epics Elite cell sorter with 4 lasers, and EPICS XL and a Bio-Rad Bryte analyzer which has an arc lamp for broad spectrum excitation. The facility provides a variety of data analysis options including Winlist listmode software, Modfit DNA cell cycle analysis software as well as routine analytical tools. The core also provides sorting capacity for -physical isolation of cells using the cell-sorter. This can be achieved under sterile conditions for subsequent culture of cells when necessary. Cancer center members also use the general laboratory facilities for preparation of material when this is necessary. Image analysis facilities include a cooled color CCD camera for fluorescence imaging, as well as an image intensified CCD for low light imaging, Metamorph and Optimus software, an MRC 1024 confocal microscope and a DVC 250 linescanner confocal microscope. A high quality fluorescence photomicroscope as well as optics for DIC and phase are available at all times in the facility. The shared resource is capable of addressing virtually any aspect of either flow cytometry or confocal microscopy available today. Cancer Center members may access the facility at any time by signing up for specific instruments. No other flow cytometry facilities are available on the Purdue campus. Although total use of the facility by Cancer Center users is modest, it serves a critical role in many research projects and is essential for further development of cancer research related to cell biology and signaling.

分析细胞学共享资源为癌症中心成员提供流式细胞术、图像分析和共聚焦显微镜设施。流式细胞术设施包括配备 4 个激光器的 Epics Elite 细胞分选仪、EPICS XL 和配备用于广谱激发的弧光灯的 Bio-Rad Bryte 分析仪。该设施提供多种数据分析选项，包括 Winlist listmode 软件、Modfit DNA 细胞周期分析软件以及常规分析工具。该核心还提供使用细胞分选器对细胞进行物理分离的分选能力。这可以在无菌条件下实现，以便必要时随后进行细胞培养。必要时，癌症中心成员也会使用一般实验室设施来准备材料。图像分析设施包括用于荧光成像的冷却彩色 CCD 相机、用于低光成像的图像增强 CCD、Metamorph 和 Optimus 软件、MRC 1024 共焦显微镜和 DVC 250 线扫描共焦显微镜。该设施随时提供高质量荧光显微镜以及 DIC 和相位光学器件。共享资源几乎能够解决当今可用的流式细胞术或共聚焦显微镜的任何方面。癌症中心成员可以通过注册特定仪器随时访问该设施。普渡大学校园内没有其他流式细胞仪设施。尽管癌症中心用户对该设施的总体使用量不大，但它在许多研究项目中发挥着关键作用，并且对于细胞生物学和信号传导相关癌症研究的进一步发展至关重要。