Core--Analytical cytology

分析细胞学共享资源为癌症中心成员提供流式细胞术、图像分析和共聚焦显微镜设施。流式细胞仪设备包括带有4个激光的EPICS Elite细胞分选仪、EPICS XL和Bio-Rad Bryte分析仪，该分析仪具有用于广谱激发的弧光灯。该设施提供各种数据分析选项，包括Winlist List模式软件、modfit DNA细胞周期分析软件以及常规分析工具。该核心还提供使用细胞分选器对细胞进行物理隔离的分选能力。这可以在无菌条件下实现，以便在必要时进行后续细胞培养。癌症中心成员还在必要时使用普通实验室设施进行材料制备。图像分析设施包括一台用于荧光成像的冷却彩色CCD相机，以及一台用于微光成像的图像增强型CCD、Metamorph和Optimus软件、一台MRC1024共焦显微镜和一台DVC 250线扫描仪共焦显微镜。高品质的荧光显微镜以及用于DIC和相位的光学装置在该设施中随时可用。共享的资源几乎能够解决当今可用的流式细胞术或共聚焦显微镜的任何方面。癌症中心的成员可以随时通过注册购买特定的仪器来访问该设施。普渡大学校园里没有其他的流式细胞仪设备。虽然癌症中心用户对该设备的总使用量不大，但它在许多研究项目中发挥着关键作用，对与细胞生物学和信号传递相关的癌症研究的进一步发展至关重要。

The Analytical Cytology Shared Resource provides flow cytometry, image analysis and confocal microscopy facilities to Cancer Center members. The flow cytometry facilities include an Epics Elite cell sorter with 4 lasers, and EPICS XL and a Bio-Rad Bryte analyzer which has an arc lamp for broad spectrum excitation. The facility provides a variety of data analysis options including Winlist listmode software, Modfit DNA cell cycle analysis software as well as routine analytical tools. The core also provides sorting capacity for -physical isolation of cells using the cell-sorter. This can be achieved under sterile conditions for subsequent culture of cells when necessary. Cancer center members also use the general laboratory facilities for preparation of material when this is necessary. Image analysis facilities include a cooled color CCD camera for fluorescence imaging, as well as an image intensified CCD for low light imaging, Metamorph and Optimus software, an MRC 1024 confocal microscope and a DVC 250 linescanner confocal microscope. A high quality fluorescence photomicroscope as well as optics for DIC and phase are available at all times in the facility. The shared resource is capable of addressing virtually any aspect of either flow cytometry or confocal microscopy available today. Cancer Center members may access the facility at any time by signing up for specific instruments. No other flow cytometry facilities are available on the Purdue campus. Although total use of the facility by Cancer Center users is modest, it serves a critical role in many research projects and is essential for further development of cancer research related to cell biology and signaling.