QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETER

请求提供资金，用于购买一台配有四极滤质器、碰撞室和飞行时间分析仪的集成高效液相色谱-电喷雾电离质谱仪。该仪器将位于生命科学质谱联盟（MSCLS）的设施中，该设施位于明尼苏达大学校园Gortner生物化学实验室43和76室新改建的2100平方英尺的空间内。该设施为生命科学提供质谱服务超过27年，完成了52，000多个样本。该仪器将高度专注于由NIH资助的研究人员在该大学分析蛋白质和核酸样品。其他时间将根据内部监督委员会的政策分配给这个大型校园的其他调查人员。用户组包括来自健康科学研究许多领域的15名研究人员。拟议研究的例子包括：“光合作用中间体和离子通道”，“脂肪酸结合蛋白的结构-功能研究”，“蛋白质与端粒的复合物”，“蚊子的分子生物学”，“细胞内基于细胞骨架的运输”，“肌肉中FK 506结合蛋白12（FKBP 12）的研究”，“维生素K依赖性蛋白”。致病性E.大肠杆菌中erbB 2的蛋白质磷酸化对IL 6的反应，雌激素对真核基因表达的调节，生物合成途径的分子育种，果蝇发育中的同源异型基因编码，肌肉收缩和钙泵作用，致癌物修饰的DNA的测序。“主要用户组估计需要80%的仪器时间。所要求的仪器是为高灵敏度和高分辨率而设计的，用于鉴定复杂生物混合物中的肽或从凝胶色谱图洗脱后的肽。高分辨率适用于MS和MS/MS模式，使得四极杆飞行时间质谱仪对于许多复杂的实验至关重要，例如从头肽测序和未知蛋白质的鉴定。还需要一个用于小样品应用的辅助纳米喷雾HPLC系统。所要求的仪器对于非共价大分子复合物的新兴领域也至关重要。

Funds are requested for purchase of an integrated HPLC-Electrospray ionization mass spectrometer with a quadrupole mass filter, a collision chamber and a time of flight analyzer. The instrument will be located in the facilities of the Mass Spectrometry Consortium for the Life Sciences (MSCLS) located in 2100 square feet of newly remodeled space in rooms 43 and 76 of Gortner Laboratories of Biochemistry on the University of Minnesota campus. This facility has provided mass spectrometry services to the life sciences for over 27 years with more than 52,000 samples completed. This instrument will be highly focused on analysis of protein and nucleic acid samples by NIH-funded researchers at the University. Any other time will be distributed among other investigators on this large campus by policies of the internal supervisory committee. The user group includes 15 investigators from many areas of health science research. Examples of proposed research include: "Photosynthetic intermediates and ion channels," "Structure- Function Studies of Fatty Acid Binding Proteins," "Protein Complexes with Telomeres," "Molecular biology of mosquitoes," "Cytoskeleton- based transport within the cell," "Study of FK506 Binding Protein 12 (FKBP12) in muscle," "Vitamin K-dependent proteins. Porins of pathogenic E. coli," "Protein Phosphorylation of erbB2 in response to IL6," "Regulation of Eucaryotic gene expression by estrogen," "Molecular breeding of biosynthetic pathways," "Homeotic genes of the fruit fly encode in development," "Muscle contraction and calcium pump action," and "Sequencing of carcinogen-modified DNA." The primary user group is estimated to require 80% of instrument time. The instrument that is requested was designed for high sensitivity and high resolution in identification of peptides in complex biological mixtures or after elution from gel electrophoretograms. High resolution applies to both the MS and MS/MS modes, making the quadrupole time of flight mass spectrometer critical for many complex experiments such as de novo peptide sequencing and identification of unknown proteins. An accessory nanospray HPLC system for application of small samples is also requested. The instrumentation that is requested is also critical for the emerging area of non-covalent macromolecular complexes.