QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETER

四极杆飞行时间质谱仪

• 批准号: 6291387
• 负责人: Gary L Nelsestuen
• 金额: $ 42.88万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291387
• 关键词: biomedical equipment purchase; mass spectrometry

Funds are requested for purchase of an integrated HPLC-Electrospray ionization mass spectrometer with a quadrupole mass filter, a collision chamber and a time of flight analyzer. The instrument will be located in the facilities of the Mass Spectrometry Consortium for the Life Sciences (MSCLS) located in 2100 square feet of newly remodeled space in rooms 43 and 76 of Gortner Laboratories of Biochemistry on the University of Minnesota campus. This facility has provided mass spectrometry services to the life sciences for over 27 years with more than 52,000 samples completed. This instrument will be highly focused on analysis of protein and nucleic acid samples by NIH-funded researchers at the University. Any other time will be distributed among other investigators on this large campus by policies of the internal supervisory committee. The user group includes 15 investigators from many areas of health science research. Examples of proposed research include: "Photosynthetic intermediates and ion channels," "Structure- Function Studies of Fatty Acid Binding Proteins," "Protein Complexes with Telomeres," "Molecular biology of mosquitoes," "Cytoskeleton- based transport within the cell," "Study of FK506 Binding Protein 12 (FKBP12) in muscle," "Vitamin K-dependent proteins. Porins of pathogenic E. coli," "Protein Phosphorylation of erbB2 in response to IL6," "Regulation of Eucaryotic gene expression by estrogen," "Molecular breeding of biosynthetic pathways," "Homeotic genes of the fruit fly encode in development," "Muscle contraction and calcium pump action," and "Sequencing of carcinogen-modified DNA." The primary user group is estimated to require 80% of instrument time. The instrument that is requested was designed for high sensitivity and high resolution in identification of peptides in complex biological mixtures or after elution from gel electrophoretograms. High resolution applies to both the MS and MS/MS modes, making the quadrupole time of flight mass spectrometer critical for many complex experiments such as de novo peptide sequencing and identification of unknown proteins. An accessory nanospray HPLC system for application of small samples is also requested. The instrumentation that is requested is also critical for the emerging area of non-covalent macromolecular complexes.

需要资金购买带有四极滤质器、碰撞室和飞行时间分析仪的集成 HPLC-电喷雾电离质谱仪。该仪器将安装在生命科学质谱联盟 (MSCLS) 的设施内，该联盟位于明尼苏达大学校园 Gortner 生物化学实验室 43 号和 76 号室新改建的 2100 平方英尺空间内。该设施已为生命科学领域提供质谱服务超过 27 年，已完成超过 52,000 个样品。该仪器将高度专注于由 NIH 资助的大学研究人员对蛋白质和核酸样本进行分析。其他时间将根据内部监督委员会的政策分配给这个大校园的其他调查员。用户组包括来自健康科学研究多个领域的 15 名研究人员。拟议研究的例子包括：“光合作用中间体和离子通道”、“脂肪酸结合蛋白的结构功能研究”、“端粒蛋白复合物”、“蚊子的分子生物学”、“细胞内基于细胞骨架的运输”、“肌肉中 FK506 结合蛋白 12 (FKBP12) 的研究”、“维生素 K 依赖性” 蛋白质。致病性大肠杆菌的孔蛋白”、“erbB2 响应 IL6 的蛋白质磷酸化”、“雌激素对真核基因表达的调节”、“生物合成途径的分子育种”、“果蝇发育中的同源基因编码”、“肌肉收缩和钙泵作用”以及“致癌物修饰 DNA 的测序”。主要用户组估计需要 80% 的仪器时间。所需的仪器设计用于识别复杂生物混合物中或凝胶电泳洗脱后的肽，具有高灵敏度和高分辨率。高分辨率适用于 MS 和 MS/MS 模式，使得四极杆飞行时间质谱仪对于许多复杂的实验（例如从头肽测序和未知蛋白质的鉴定）至关重要。还需要用于小样品应用的附件纳喷雾 HPLC 系统。所需的仪器对于非共价大分子复合物的新兴领域也至关重要。

项目成果

Characterizing the Epothilone Binding Site on β-Tubulin by Photoaffinity Labeling: Identification of β-Tubulin Peptides TARGSQQY and TSRGSQQY as Targets of an Epothilone Photoprobe for Polymerized Tubulin.

• DOI: 10.1021/acs.jmedchem.6b00188
• 发表时间: 2016-04-14
• 期刊: Journal of medicinal chemistry
• 影响因子: 7.3
• 作者: Ranade AR;Higgins L;Markowski TW;Glaser N;Kashin D;Bai R;Hong KH;Hamel E;Höfle G;Georg GI
• 通讯作者: Georg GI