CORE--HUMAN AIRWAY CELL AND TISSUE

核心——人体气道细胞和组织

• 批准号: 6358026
• 负责人: JOSEPH Mark PILEWSKI
• 金额: $ 14.22万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6358026
• 关键词: biomedical facility; chloride channels; cystic fibrosis; genotype; human tissue; respiratory epithelium; tissue /cell culture; tissue resource /registry

The purpose of the Human Airway Cell and Tissue ore is to provide human airway cell models and establish a bank of Cystic Fibrosis (CF) and non- Cystic Fibrosis (non-CF) lung tissue for a number of research projects within the Cystic Fibrosis Research CENTER. Accordingly, the specific aims are: 1. To establish primary cultures of human airway epithelium from cystic fibrosis and non-cystic fibrosis lung for studies of CFTR function in F and non-CF airway. Studies of CFTR function are best performed in human airway epithelium. To create a laboratory model of human airway, cells derived from human airway are grown in the laboratory on permeable supports to induce differentiation and recapitulate airway surface epithelium in vitro. This model will be used for Center projects that seek to evaluate the cellular physiology and mechanisms of membrane trafficking of CFTR. 2. To determine the genotype of the CF airway epithelial cells. To permit correlation of genotype with drug-induced changes in ion transport and CFTR function in primary airway cells, mutation analysis for the 70 most common CF mutations will be obtained from a reference laboratory. 3. To create a frozen bank of CF and non-CF airway cells and airway tissue. Isolated airway cells and segments of dissected lung will be frozen with and without prior fixation to establish a cell and tissue bank for future use that attempts to understand more about how mutations in the CF gene cause lung disease and allow comparison of mRNA and protein expression in CF and non-CF lung tissue.

人类气道细胞和组织矿石的目的是提供人类气道细胞模型，并建立囊性纤维化（CF）和非囊性纤维化（non-CF）肺组织库，用于囊性纤维化研究中心的一些研究项目。因此，具体目标是：1。建立人囊性纤维化和非囊性纤维化气道上皮原代培养，研究CFTR在F型和非cf型气道中的功能。CFTR功能的研究最好在人气道上皮中进行。为了建立人类气道的实验室模型，在实验室中，将来自人类气道的细胞培养在可渗透的支架上，诱导体外分化和概括气道表面上皮。该模型将用于中心项目，旨在评估CFTR的细胞生理学和膜运输机制。2. 目的：测定CF气道上皮细胞的基因型。为了将基因型与原代气道细胞中药物诱导的离子转运和CFTR功能的变化联系起来，将从参考实验室获得70种最常见的CF突变的突变分析。3. 建立CF和非CF气道细胞和气道组织的冷冻库。将分离的气道细胞和解剖肺段冷冻，事先固定或不固定，以建立细胞和组织库供将来使用，试图更多地了解CF基因突变如何导致肺部疾病，并比较CF和非CF肺组织中的mRNA和蛋白表达。