CORE--HUMAN AIRWAY CELL AND TISSUE

人类呼吸道细胞和组织ORE的目的是提供人类呼吸道细胞模型，并为囊性纤维化研究中心内的一些研究项目建立囊性纤维化(CF)和非囊性纤维化(Non-CF)肺组织库。因此，本研究的具体目的是：1.建立囊性纤维化和非囊性纤维化人肺上皮细胞原代培养体系，为研究CFTR在F和非囊性纤维化中的功能奠定基础。CFTR功能的研究最好在人的呼吸道上皮细胞中进行。为了建立人类呼吸道的实验室模型，在实验室中将人呼吸道来源的细胞培养在可渗透的支架上，以诱导分化并在体外重塑呼吸道表面上皮细胞。该模型将用于中心项目，这些项目寻求评估CFTR膜运输的细胞生理学和机制。2.确定CF型呼吸道上皮细胞的基因分型。为了使基因与药物引起的原代呼吸道细胞离子转运和CFTR功能的变化相关，将从参考实验室获得对70个最常见的CF突变的突变分析。3.建立冷冻库，由CF型和非CF型呼吸道细胞和呼吸道组织组成。分离的呼吸道细胞和解剖的肺段将在有和没有事先固定的情况下被冷冻，以建立一个细胞和组织库，以供将来使用，试图更多地了解CF基因突变是如何导致肺部疾病的，并允许比较CF和非CF肺组织中的mRNA和蛋白质表达。

The purpose of the Human Airway Cell and Tissue ore is to provide human airway cell models and establish a bank of Cystic Fibrosis (CF) and non- Cystic Fibrosis (non-CF) lung tissue for a number of research projects within the Cystic Fibrosis Research CENTER. Accordingly, the specific aims are: 1. To establish primary cultures of human airway epithelium from cystic fibrosis and non-cystic fibrosis lung for studies of CFTR function in F and non-CF airway. Studies of CFTR function are best performed in human airway epithelium. To create a laboratory model of human airway, cells derived from human airway are grown in the laboratory on permeable supports to induce differentiation and recapitulate airway surface epithelium in vitro. This model will be used for Center projects that seek to evaluate the cellular physiology and mechanisms of membrane trafficking of CFTR. 2. To determine the genotype of the CF airway epithelial cells. To permit correlation of genotype with drug-induced changes in ion transport and CFTR function in primary airway cells, mutation analysis for the 70 most common CF mutations will be obtained from a reference laboratory. 3. To create a frozen bank of CF and non-CF airway cells and airway tissue. Isolated airway cells and segments of dissected lung will be frozen with and without prior fixation to establish a cell and tissue bank for future use that attempts to understand more about how mutations in the CF gene cause lung disease and allow comparison of mRNA and protein expression in CF and non-CF lung tissue.