CORE--HUMAN AIRWAY CELL AND TISSUE

人类气道细胞和组织矿石的目的是提供人类气道细胞模型，并为囊性纤维化研究中心的许多研究项目建立囊性纤维化（CF）和非囊性纤维化（非CF）肺组织库。因此，具体目标是：1。目的建立人囊性纤维化和非囊性纤维化肺组织的原代培养细胞，研究囊性纤维化和非囊性纤维化肺组织CFTR功能。CFTR功能的研究最好在人气道上皮中进行。为了建立人气道的实验室模型，在实验室中将源自人气道的细胞生长在可渗透的支持物上以诱导分化并在体外重现气道表面上皮。该模型将用于中心项目，旨在评估CFTR的细胞生理学和膜运输机制。2.确定CF气道上皮细胞的基因型。为了使基因型与药物诱导的原代气道细胞离子转运和CFTR功能变化相关，将从参考实验室获得70种最常见CF突变的突变分析。3.建立CF和非CF气道细胞和气道组织的冷冻库。分离的气道细胞和解剖的肺段将在预先固定和不预先固定的情况下冷冻，以建立细胞和组织库供将来使用，其试图更多地了解CF基因突变如何导致肺部疾病，并允许比较CF和非CF肺组织中的mRNA和蛋白质表达。

The purpose of the Human Airway Cell and Tissue ore is to provide human airway cell models and establish a bank of Cystic Fibrosis (CF) and non- Cystic Fibrosis (non-CF) lung tissue for a number of research projects within the Cystic Fibrosis Research CENTER. Accordingly, the specific aims are: 1. To establish primary cultures of human airway epithelium from cystic fibrosis and non-cystic fibrosis lung for studies of CFTR function in F and non-CF airway. Studies of CFTR function are best performed in human airway epithelium. To create a laboratory model of human airway, cells derived from human airway are grown in the laboratory on permeable supports to induce differentiation and recapitulate airway surface epithelium in vitro. This model will be used for Center projects that seek to evaluate the cellular physiology and mechanisms of membrane trafficking of CFTR. 2. To determine the genotype of the CF airway epithelial cells. To permit correlation of genotype with drug-induced changes in ion transport and CFTR function in primary airway cells, mutation analysis for the 70 most common CF mutations will be obtained from a reference laboratory. 3. To create a frozen bank of CF and non-CF airway cells and airway tissue. Isolated airway cells and segments of dissected lung will be frozen with and without prior fixation to establish a cell and tissue bank for future use that attempts to understand more about how mutations in the CF gene cause lung disease and allow comparison of mRNA and protein expression in CF and non-CF lung tissue.