Core--Human neurobiology

CNNMD的人类神经生物学核心：自杀行为的神经生物学维持着临床神经科学脑库。这包括收集大脑样本，并对所有受试者进行全面的毒理学、神经病理学和精神病学鉴定。然后对所有组织进行不同的切片，并在不同的冠状层上放置一系列插入的玻璃切片。同样，将固定的脑干切片并保存在冷冻保护剂中，这允许系统地执行各种细胞结构学，免疫细胞化学和免疫自显影方案。人类神经生物学核心的脑库为Conte中心项目2的研究人员以及其他依赖于麸皮库组织的研究人员提供了所有的组织。它还将组织送到临床实验室核心，对所有样本进行毒理学筛选，进行基因分型（候选基因），并对临床实验室核心提出的基因表达进行微阵列检测。人类神经生物学核心提供了所有组织、放射配体和免疫细胞化学分析的标准化方案，包括项目1中提出的方案。

The Human Neurobiology Core of the CNNMD: The Neurobiology of Suicidal Behavior maintains the Clinical Neuroscience Brain Bank. This involves collection of brain samples and full toxicological, neuropathological and psychiatric characterization of all subjects. All tissues are then sectioned for the various protocols and a series of intercalated sets of glass mounted sections at various coronal levels are kept in storage. Similarly, fixed brainstems are sectioned and stored in cryoprotectant, which allows for various cytoarchitectonic, immunocytochemical and immunoautoradiographic protocols to be systematically performed. The Brain Bank of the Human Neurobiology Core provides all the tissue to the investigators in Project 2 of the Conte Center, as well as other investigators whose R01s are dependent on the tissue from the Bran Bank. It also sends tissue to the Clinical Laboratory Core for toxicological screening of all samples, for genotyping (candidate genes) and the microarray assays of gene expression proposed in the Clinical Laboratory Core. The Human Neurobiology Core provides standardized protocols for all histological, radioligand and immunocytochemical assays proposed including those proposed in Project 1.