Core--Human neurobiology

CNNMD的人类神经生物学核心：自杀行为的神经生物学维持着临床神经科学的脑库。这包括收集大脑样本和所有受试者的全部毒理学、神经病理学和精神病学特征。然后对所有组织进行不同方案的切片，并将不同冠状水平的一系列夹层玻璃安装切片保存在仓库中。同样，固定的脑组织切片并储存在冷冻保护剂中，这允许系统地执行各种细胞结构、免疫细胞化学和免疫放射自显影程序。人类神经生物学核心的脑库为孔特中心项目2的研究人员以及其他研究人员提供所有组织，这些研究人员的R01依赖于来自Bran Bank的组织。它还将组织送到临床实验室核心进行所有样本的毒理筛选、基因分型(候选基因)和临床实验室核心建议的基因表达微阵列分析。人类神经生物学核心为所有建议的组织学、放射性配基和免疫细胞化学分析提供标准化方案，包括项目1中建议的那些。

The Human Neurobiology Core of the CNNMD: The Neurobiology of Suicidal Behavior maintains the Clinical Neuroscience Brain Bank. This involves collection of brain samples and full toxicological, neuropathological and psychiatric characterization of all subjects. All tissues are then sectioned for the various protocols and a series of intercalated sets of glass mounted sections at various coronal levels are kept in storage. Similarly, fixed brainstems are sectioned and stored in cryoprotectant, which allows for various cytoarchitectonic, immunocytochemical and immunoautoradiographic protocols to be systematically performed. The Brain Bank of the Human Neurobiology Core provides all the tissue to the investigators in Project 2 of the Conte Center, as well as other investigators whose R01s are dependent on the tissue from the Bran Bank. It also sends tissue to the Clinical Laboratory Core for toxicological screening of all samples, for genotyping (candidate genes) and the microarray assays of gene expression proposed in the Clinical Laboratory Core. The Human Neurobiology Core provides standardized protocols for all histological, radioligand and immunocytochemical assays proposed including those proposed in Project 1.