CORE--TRANSGENIC ANIMAL SUPPORT

描述：总部设在比较医学系的转基因动物服务中心提供了一个校园单元，CEEH的研究人员可以在这里提交他们的构建物或目标胚胎干细胞，以产生转基因或基因靶向的小鼠品系。该中心的具体目标是：1)为在与中心主题相关的研究中使用或打算使用转基因动物的CEEH研究人员提供资源，以抵消开发转基因动物品系的高昂成本；2)在与中心主题相关的研究中部分支持维护和使用转基因动物品系；以及3)为希望建立自己的转基因动物的缺乏经验的CEEH研究人员提供有限的咨询、建议和培训。该中心目前提供的服务和资源包括：1)通过显微注射和胚胎干细胞靶向突变产生转基因和基因靶向小鼠；2)冷冻保存129和C57BL/6背景的小鼠ES细胞系库存；3)管理基因工程小鼠繁殖群体；4)在必要时保持基因工程小鼠不并发疾病或获得感染菌株；5)就载体设计和选择合适的小鼠背景品系等事宜提供咨询。沃伦·拉迪吉斯博士自1986年以来一直担任核心中心主任。

Description: The Transgenic Animal Services Core, based in the Department of Comparative Medicine, provides an on-campus unit where CEEH investigators can submit their constructs or their targeted embryonic stem (ES) cells to generate transgenic or gene-targeted mouse lines. The specific aims of the Core are: 1) provide resources to offset the high costs of developing transgenic animal strains for CEEH investigators who use, or intend to use, transgenic animals in research related to the Center's theme; 2) partially support maintenance and use of transgenic animal lines in research related to the Center's theme; and 3) provide limited consultation, advice, and training to inexperienced CEEH investigators wishing to establish their own transgenic animals. Services and resources currently provided by the Core include: 1) generation of transgenic and gene-targeted mice by microinjection and embryonic stem (ES) cell-targeted mutagenesis, 2) cryopreserved stocks of mouse ES cell lines of the 129 and C57BL/6 backgrounds, 3) management of genetically engineered mouse breeding colonies, 4) maintenance of genetically engineered mice free of intercurrent disease or derivation of infected strains when necessary, and 5) consultation with regard to such matters as vector design and selections of appropriate mouse background strains. Dr. Warren Ladiges has served as director of the Core since 1986.