MOLECULAR EPIDEMIOLOGY OF PERSISTENT HPV INFECTION

描述：这是一个继续研究的竞争性继续申请 人乳头瘤病毒（HPV）生殖器持久性在一个大的队列低 巴西圣保罗的高收入妇女是宫颈癌的高危地区。的 研究的重点是致癌HPV类型对发展的关系， 宫颈上皮病变和癌。参与者将经历一系列 基于性别的访谈，以了解性行为和其他潜在的 影响因素，PAP涂片，HPV PCR检测，HPV血清学和 宫颈造影其目标是 1 宫颈HPV持续感染发生率和患病率研究 无症状妇女; 2 验证持续性HPV增加低和高的假设 宫颈病变分级; 3 检测HPV持续存在的流行病学决定因素; 4 寻找与宫颈病变相关的分子变异; 5 为了确定HPV病毒载量是否与持久性相关， 以及低或高级别病变;以及 6 研究抗体应答在HPV持续存在和病变中的作用 进展 对于竞争性更新，提出了2个额外目标： 7 与HPV持续感染和宫颈癌相关HLA单倍型 病变;以及 8 测试p53多态性是否赋予对HPV持久性的抗性 和病变发展。

DESCRIPTION: This is a competing continuation application for continued study of human papilloma virus (HPV) genital persistent in a large cohort of low income women in Sao Paolo, Brazil, a high risk area for cervical carcinoma. The study focuses on the relationship of oncogenic HPV types on the development of cervical epithelial lesions and carcinoma. Participants undergo a series of questionnaire-based interviews to access sexual behavior and other potentially contributing factors, PAP smears, HPV testing by PCR, HPV serology and cervicography. The objectives are to 1 study incidence and prevalence of persistent cervical HPV in asymptomatic women; 2 verify the hypothesis that persistent HPV increases low and high grade cervical lesions; 3 detect epidemiological determinants of HPV persistence; 4 search for molecular variants associated with cervical lesions; 5 to determine whether HPV viral load is associated with persistence, and low or high grade lesions; and 6 study the role of antibody response to HPV persistence and lesion progression. For the competitive renewal, 2 additional aims are proposed: 7 identity HLA haplotypes associated with HPV persistence and cervical lesions; and 8 test whether p53 polymorphisms confer resistance to HPV persistence and lesion development.