CELL ADHESION ON PROTEIN-MICROPATTERNED LIPID BILAYERS

蛋白质微图案脂质双层上的细胞粘附

• 批准号: 6298612
• 负责人: Lance C Kam
• 金额: $ 3.48万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6298612
• 关键词: bioengineering /biomedical engineering; biomaterial development /preparation; biomaterial interface interaction; biomedical equipment development; biosensor device; biotechnology; cell adhesion; cell cell interaction; fluorescence microscopy; gap junctions; lipid bilayer membrane; membrane activity; membrane proteins; membrane reconstitution /synthesis; membrane structure

The goal of this proposed research is to develop micropatterned lipid bilayers into a mature system with great potential in cellular bioengineering. In preliminary feasibility studies, I discovered a technique for creating multi functionalized surfaces containing microscale patterns of cell-adhesive proteins, surround by region of lipid bilayers. These barriers facilitated the spreading of anchorage-dependent cells across lipid bilayers, overcoming a fundamental obstacle in the use of these supported membranes to study and modulate cell response. The propose research will use contemporary surface analysis and molecular biophysical approaches to characterize these newly developed surfaces ,providing the understanding needed to effectively manipulate this system. The ability of adherent endothelial cells to interact with bilayer-associated biomolecular targets, including lipid-tethered peptides and membrane spanning proteins involved in cell-cell communication, will also be examined. These surfaces will be used to identify specific membrane properties that regulate cellular function, leading to new techniques in biomaterials engineering. Successful completion of the goals set forth in this study will also provide tools needed to develop interfaces between cells and biosensors in vitro, and will culminate in the design of a proposed interface based on membrane proteins and supported lipid bilayers.

本研究的目标是将微图案化的脂质双层发展成一个成熟的系统，在细胞生物工程中具有巨大的潜力。在初步的可行性研究中，我发现了一种技术，用于创建包含细胞粘附蛋白的微尺度图案的多功能表面，周围是脂质双层区域。这些障碍促进了跨脂质双层的锚定依赖性细胞的扩散，克服了使用这些支持膜来研究和调节细胞反应的根本障碍。这项研究将使用当代表面分析和分子生物物理方法来表征这些新开发的表面，提供有效操作该系统所需的理解。还将检查粘附内皮细胞与双层相关生物分子靶标（包括参与细胞-细胞通信的脂质栓系肽和跨膜蛋白）相互作用的能力。这些表面将用于识别调节细胞功能的特定膜特性，从而为生物材料工程带来新技术。在这项研究中提出的目标的成功完成也将提供所需的工具，开发细胞和生物传感器之间的接口在体外，并将最终在一个拟议的界面的设计基于膜蛋白和支持脂质双层。