CELL ADHESION ON PROTEIN-MICROPATTERNED LIPID BILAYERS

蛋白质微图案脂质双层上的细胞粘附

• 批准号: 6518905
• 负责人: Lance C Kam
• 金额: $ 2.49万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6518905
• 关键词: bioengineering /biomedical engineering; biomaterial development /preparation; biomaterial interface interaction; biomedical equipment development; biosensor device; biotechnology; cell adhesion; cell cell interaction; fluorescence microscopy; gap junctions; lipid bilayer membrane; membrane activity; membrane proteins; membrane reconstitution /synthesis; membrane structure

The goal of this proposed research is to develop micropatterned lipid bilayers into a mature system with great potential in cellular bioengineering. In preliminary feasibility studies, I discovered a technique for creating multi functionalized surfaces containing microscale patterns of cell-adhesive proteins, surround by region of lipid bilayers. These barriers facilitated the spreading of anchorage-dependent cells across lipid bilayers, overcoming a fundamental obstacle in the use of these supported membranes to study and modulate cell response. The propose research will use contemporary surface analysis and molecular biophysical approaches to characterize these newly developed surfaces ,providing the understanding needed to effectively manipulate this system. The ability of adherent endothelial cells to interact with bilayer-associated biomolecular targets, including lipid-tethered peptides and membrane spanning proteins involved in cell-cell communication, will also be examined. These surfaces will be used to identify specific membrane properties that regulate cellular function, leading to new techniques in biomaterials engineering. Successful completion of the goals set forth in this study will also provide tools needed to develop interfaces between cells and biosensors in vitro, and will culminate in the design of a proposed interface based on membrane proteins and supported lipid bilayers.

本研究的目标是将微模式脂质双分子层发展成为一个成熟的系统，在细胞生物工程中具有很大的潜力。在初步的可行性研究中，我发现了一种创建多功能表面的技术，该表面包含细胞粘附蛋白的微尺度模式，周围是脂质双层区域。这些屏障促进了锚定依赖细胞在脂质双分子层上的扩散，克服了使用这些支撑膜研究和调节细胞反应的基本障碍。该研究将使用当代表面分析和分子生物物理方法来表征这些新开发的表面，提供有效操纵该系统所需的理解。粘附内皮细胞与双分子层相关生物分子靶标（包括参与细胞间通讯的脂系肽和膜跨越蛋白）相互作用的能力也将被检查。这些表面将用于识别调节细胞功能的特定膜特性，从而导致生物材料工程中的新技术。成功完成本研究设定的目标也将为在体外开发细胞和生物传感器之间的界面提供所需的工具，并最终设计基于膜蛋白和支持脂质双分子层的拟议界面。