CELL ADHESION ON PROTEIN-MICROPATTERNED LIPID BILAYERS

这项研究的目标是将微图案化的脂质双层发展成一个在细胞生物工程中具有巨大潜力的成熟系统。在初步的可行性研究中，我发现了一种创建多功能表面的技术，该表面包含细胞黏附蛋白的微尺度图案，周围是脂类双层区域。这些屏障促进了依赖于锚定的细胞跨脂双层扩散，克服了使用这些支撑膜来研究和调节细胞反应的根本障碍。这项拟议的研究将使用当代表面分析和分子生物物理方法来表征这些新开发的表面，提供有效操纵这一系统所需的理解。贴壁内皮细胞与双层相关生物分子靶点相互作用的能力也将被检测，包括参与细胞-细胞通讯的脂系多肽和跨膜蛋白。这些表面将被用来识别调节细胞功能的特定膜属性，从而在生物材料工程中引入新技术。成功完成这项研究中提出的目标还将提供在体外开发细胞和生物传感器之间接口所需的工具，并最终设计出基于膜蛋白和支持的脂类双层的拟议接口。

The goal of this proposed research is to develop micropatterned lipid bilayers into a mature system with great potential in cellular bioengineering. In preliminary feasibility studies, I discovered a technique for creating multi functionalized surfaces containing microscale patterns of cell-adhesive proteins, surround by region of lipid bilayers. These barriers facilitated the spreading of anchorage-dependent cells across lipid bilayers, overcoming a fundamental obstacle in the use of these supported membranes to study and modulate cell response. The propose research will use contemporary surface analysis and molecular biophysical approaches to characterize these newly developed surfaces ,providing the understanding needed to effectively manipulate this system. The ability of adherent endothelial cells to interact with bilayer-associated biomolecular targets, including lipid-tethered peptides and membrane spanning proteins involved in cell-cell communication, will also be examined. These surfaces will be used to identify specific membrane properties that regulate cellular function, leading to new techniques in biomaterials engineering. Successful completion of the goals set forth in this study will also provide tools needed to develop interfaces between cells and biosensors in vitro, and will culminate in the design of a proposed interface based on membrane proteins and supported lipid bilayers.