Prevention of Hip Dysplasia with Thermal Energy

用热能预防髋关节发育不良

• 批准号: 6324250
• 负责人: MANDI J. LOPEZ
• 金额: $ 10.26万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6324250
• 关键词: articular cartilage; biomarker; biomechanics; chondroitin sulfates; collagen; computed axial tomography; disease /disorder prevention /control; dogs; enzyme linked immunosorbent assay; gene expression; hip; hyperthermia therapy; joint disorder; longitudinal animal study; mucopolysaccharides; nonhuman therapy evaluation; osteoarthritis; polymerase chain reaction; proteoglycan; radiography; radioimmunoassay; radiowave radiation; synovial fluid; viscosity

The fundamental goals of this research proposal are to test the hypotheses that: 1) application of thermal energy at 65 degrees C using radio-frequency energy to the coxofemoral joint capsule of puppies with radiographic evidence of joint laxity will result in normal hip congruity, 2) will prevent the development of the lameness and the cartilagenous and bony changes characteristic of CHD, and 3) will not have detrimental effects on the biomechanical or viscoelastic properties of the joint capsular tissue. These investigations will yield insights into the fundamental mechanisms of thermal modification of musculoskeletal soft tissue. This research program is designed to determine whether thermal modification of coxocofemoral joint capsule of 16-week-old puppies with excessive joint: laxity will prevent CHD. Ultimately, this model will serve to evaluate the potential of thermal energy to reduce excessively lax coxofemoral joints in neonatal children and thereby help prevent DDH. The candidate of this application graduated magna cum laude from Humboldt State University with a B.S. in cellular-molecular biology and a minor in chemistry. She worked as a molecular biologist at Amgen, Thousand Oaks, CA, before undertaking and; completing her D.V.M. at the University of California-Davis. Dr. Lopez was awarded 5 scholarships and 2 research grants while a D.V.M. student. She completed an internship at Kansas State University and a large animal surgery residency/Master's degree at they University of Wisconsin- Madison. She is a board certified large animal surgeon. Dr. Lopez's Master's thesis was entitled "Effects of Radio-frequency Energy on Ovine Stifle Joint Capsule", and she performed much of the early radio- frequency work in the Comparative Orthopaedic Research Laboratory. She was awarded an NRSA in 1998 and has performed the background research and preliminary, studies that form the basis for this proposal. She passed her preliminary examinations "With Honors" in November, 1999, and is scheduled to complete her PhD in June 2001. Her ultimate goal is to join the faculty of an academic institution as a well-trained scientist and productive researcher specializing in integrative, translational research. The training environment will be guided toward mentoring the candidate while teaching valuable research techniques necessary to begin an independent research career. The majority of supervision will be provided by Mark D. Markel, an internationally known and respected investigator in the area of thermal tissue modification, Professor of Surgery, and Professor and Chairman of Medical Sciences. The advisory committee will consist of well-respected and highly experienced individuals within the University. The Comparative Orthopaedic Research Laboratory is a well established laboratory that houses multi- disciplinary research programs. The University of Wisconsin provides an exceptional environment for interaction with experts in a variety of fields, and it is well known for its exceptional research programs, especially in the medical sciences.

本研究提案的基本目标是检验以下假设：1）使用射频能量在65 ℃下对具有关节松弛的放射学证据的幼犬的髋股关节囊施加热能将导致正常的髋关节一致性，2）将防止跛行的发展以及CHD特征性的软骨和骨变化，和3）不会对关节囊组织的生物力学或粘弹性产生有害影响。这些调查将深入了解肌肉骨骼软组织的热改性的基本机制。这项研究计划旨在确定是否热修改的髋股关节囊的16周大的小狗与过度关节：松弛将防止冠心病。最终，该模型将用于评估热能减少新生儿髋股关节过度松弛的潜力，从而有助于预防DDH。本申请的候选人以优异成绩毕业于洪堡州立大学，获得学士学位。主修细胞分子生物学辅修化学她在加利福尼亚州千橡市安进公司担任分子生物学家，之后开始从事并完成了D.V.M.。在加州大学戴维斯分校洛佩斯博士获得了5个奖学金和2个研究赠款，而D.V.M.学生.她在堪萨斯州立大学完成了实习，并在威斯康星州-麦迪逊大学获得了大型动物外科住院医师/硕士学位。她是一名获得认证的大型动物外科医生。洛佩斯博士的硕士论文题目是“射频能量对羊颈关节囊的影响”，她在比较矫形研究实验室进行了许多早期的射频工作。她于1998年被授予NRSA，并进行了背景研究和初步研究，这些研究构成了这一建议的基础。她于1999年11月以优异成绩通过了初步考试，并计划于2001年6月完成博士学位。她的最终目标是加入一个学术机构的教师作为一个训练有素的科学家和生产力的研究人员专门从事综合，转化研究。培训环境将指导指导候选人，同时教授开始独立研究生涯所需的宝贵研究技术。大部分的监督工作将由Mark D提供。Markel是热组织修饰领域的国际知名和受人尊敬的研究者，外科教授，医学科学教授兼主席。咨询委员会将由大学内备受尊敬和经验丰富的人士组成。比较矫形研究实验室是一个完善的实验室，容纳多学科的研究计划。威斯康星州的大学提供了一个特殊的环境与专家在各个领域的互动，它是众所周知的，其特殊的研究计划，特别是在医学科学。